3.4.22.32: Stem bromelain
This is an abbreviated version!
For detailed information about Stem bromelain, go to the full flat file.
Word Map on EC 3.4.22.32
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3.4.22.32
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papain
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ficin
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comosus
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chymotrypsin
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ananas
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hydrolysates
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allergen
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proteinases
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ige
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debridement
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neuraminidase
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alcalase
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cystatins
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virion
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hymenoptera
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flavourzyme
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medicine
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kininogens
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prick
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eschar
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tender
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haemagglutinins
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food industry
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actinidin
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ha2
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agriculture
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chymopapain
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mucolytic
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pharmacology
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pancreatin
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ige-binding
- 3.4.22.32
- papain
- ficin
- comosus
- chymotrypsin
- ananas
- hydrolysates
- allergen
- proteinases
- ige
-
debridement
- neuraminidase
- alcalase
- cystatins
- virion
- hymenoptera
- flavourzyme
- medicine
- kininogens
-
prick
-
eschar
-
tender
-
haemagglutinins
- food industry
- actinidin
- ha2
- agriculture
- chymopapain
-
mucolytic
- pharmacology
- pancreatin
-
ige-binding
Reaction
broad specificity for cleavage of proteins, but strong preference for Z-Arg-Arg-/-NHMec amongst small molecule substrates =
Synonyms
acidic bromelain stem proteinase, ACMD2_17643, BRM, Bromelain, Bromelain, stem, EC 3.4.22.4, EC 3.4.4.24, Pineapple stem bromelain, SBA, Sbm, stem bromelain
ECTree
3.4.22.32 Stem bromelainAdvanced search results
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results (KM Value
KM Value on EC 3.4.22.32 - Stem bromelain
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.249
benzyl-Phe-Val-Arg-4-nitroanilide
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tartaric buffer containing 12% ethanol, pH 3.2, 25°C, stem bromelain
200
benzoyl-L-Arg ethyl ester
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enzyme form SB2, benzoyl-Gly ethyl ester, enzyme form SB1 and SB2
0.0074
benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide
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isozyme SBA/b, pH 6.0, 25°C
0.0081
benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide
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isozyme SBA/a, pH 6.0, 25°C
0.0554
benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide
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isozyme SBA/a, pH 6.0, 25°C
0.0636
benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide
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isozyme SBA/b, pH 6.0, 25°C
0.003
benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide
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isozyme SBA/b, pH 6.0, 25°C
0.0039
benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide
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isozyme SBA/a, pH 6.0, 25°C
0.03919
Bz-Phe-Val-Arg-4-nitroanilide
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chitosan-immobilized enzyme, at pH 3.2 and 25°C
0.0495
Bz-Phe-Val-Arg-4-nitroanilide
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chitosan-immobilized enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C
0.05731
Bz-Phe-Val-Arg-4-nitroanilide
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chitosan-immobilized enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C
0.25
Bz-Phe-Val-Arg-4-nitroanilide
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free enzyme, in the presence of 12% (v/v) ethanol, at pH 3.2 and 25°C
0.305
Bz-Phe-Val-Arg-4-nitroanilide
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free enzyme, in the presence of 18% (v/v) ethanol, at pH 3.2 and 25°C
2.83
casein
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poly(maleic anhydride)-modified enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 40°C
4.09
casein
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pyromellitic anhydride-modified enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 40°C
additional information
additional information
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kinetics of hydrolysis and influence of modifiers of hydrolysis of N-benzoyl-L-Ser methyl ester
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additional information
additional information
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kinetics of reaction of benzofuroxan, a thiol-specific reactivity probe with the enzyme
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additional information
additional information
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Km-for casein is 1.1 mg/ml for the native enzyme, 2 mg/ml for the enzyme covalently coupled to the CNBr-activated Sepharose and 0.54 mg/ml for enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A
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