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Literature summary for 3.4.22.32 extracted from

  • Rowan, A.D.; Buttle, D.J.; Barrett, A.J.
    Ananain: a novel cysteine proteinase found in pineapple stem (1988), Arch. Biochem. Biophys., 267, 262-270.
    View publication on PubMed
Search for more literature for 3.4.22.32

Inhibitors

Inhibitors Comment Organism Structure
L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane irreversible Ananas comosus
additional information scarcely inhibited by chicken cystatin (amino acid sequence and implications for weak binding of cystatin) Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0154
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
0.0831
-
 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, Ananas comosus, SDS-PAGE Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, Ananas comosus, SDS-PAGE Ananas comosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
27
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus