3.4.22.32: Stem bromelain
This is an abbreviated version!
For detailed information about Stem bromelain, go to the full flat file.
Word Map on EC 3.4.22.32
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3.4.22.32
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papain
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ficin
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comosus
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chymotrypsin
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ananas
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hydrolysates
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allergen
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proteinases
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ige
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debridement
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neuraminidase
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alcalase
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cystatins
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virion
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hymenoptera
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flavourzyme
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medicine
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kininogens
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prick
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eschar
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tender
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haemagglutinins
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food industry
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actinidin
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ha2
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agriculture
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chymopapain
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mucolytic
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pharmacology
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pancreatin
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ige-binding
- 3.4.22.32
- papain
- ficin
- comosus
- chymotrypsin
- ananas
- hydrolysates
- allergen
- proteinases
- ige
-
debridement
- neuraminidase
- alcalase
- cystatins
- virion
- hymenoptera
- flavourzyme
- medicine
- kininogens
-
prick
-
eschar
-
tender
-
haemagglutinins
- food industry
- actinidin
- ha2
- agriculture
- chymopapain
-
mucolytic
- pharmacology
- pancreatin
-
ige-binding
Reaction
broad specificity for cleavage of proteins, but strong preference for Z-Arg-Arg-/-NHMec amongst small molecule substrates =
Synonyms
acidic bromelain stem proteinase, ACMD2_17643, BRM, Bromelain, Bromelain, stem, EC 3.4.22.4, EC 3.4.4.24, Pineapple stem bromelain, SBA, Sbm, stem bromelain
ECTree
3.4.22.32 Stem bromelainAdvanced search results
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results (Temperature Stability
Temperature Stability on EC 3.4.22.32 - Stem bromelain
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
100
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recombinant bromelain shows no activity after 30 min of incubation at 100°C
4
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recombinant protein stored at 4°C shows a loss in enzymatic activity. The percentage loss in recombinant bromelain enzymatic activity is 27.5%
45
60
70
soluble enzyme retains 53% activity after incubation at 70°C, immobilized enzyme retains over 70% activity after incubation at 70°C
additional information
60
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100 min, native enzyme loses 80% of initial activity, enzyme covalently coupled to the CNBr-activated Sepharose loses 70% of the initial activity, enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A loses 50% of initial activity
60
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native bromelain rapidly loses activity at 60°C and retains only about 10% of the initial activity after incubation for 180 min, about 70% and 58% of the initial activity is retained by uniformly coupled and randomly coupled N-isopropylacrylamide-enzyme preparations, respectively, after 180 min incubation at 60°C
60
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the native enzyme retains 20% of the initial activity after incubation at 60°C
additional information
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thermal denaturation is consistent with an irreversible two-state model
additional information
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immobilization offers more resistance to denaturation particularly at 60°C
