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3.4.22.32: Stem bromelain

This is an abbreviated version!
For detailed information about Stem bromelain, go to the full flat file.

Word Map on EC 3.4.22.32

Reaction

broad specificity for cleavage of proteins, but strong preference for Z-Arg-Arg-/-NHMec amongst small molecule substrates =

Synonyms

acidic bromelain stem proteinase, ACMD2_17643, BRM, Bromelain, Bromelain, stem, EC 3.4.22.4, EC 3.4.4.24, Pineapple stem bromelain, SBA, Sbm, stem bromelain

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.32 Stem bromelain

Cloned

Cloned on EC 3.4.22.32 - Stem bromelain

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the pENTR/TEV/D-TOPO vector, then sub-cloned into the pDEST17 expression vector. Expression in Escherichia coli BL21
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