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3.4.22.32: Stem bromelain

This is an abbreviated version!
For detailed information about Stem bromelain, go to the full flat file.

Word Map on EC 3.4.22.32

Reaction

broad specificity for cleavage of proteins, but strong preference for Z-Arg-Arg-/-NHMec amongst small molecule substrates =

Synonyms

acidic bromelain stem proteinase, ACMD2_17643, BRM, Bromelain, Bromelain, stem, EC 3.4.22.4, EC 3.4.4.24, Pineapple stem bromelain, SBA, Sbm, stem bromelain

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.32 Stem bromelain

Molecular Weight

Molecular Weight on EC 3.4.22.32 - Stem bromelain

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
-
Ananas comosus, enzyme form I-2, II-1 and II-2, gel filtration
18800
-
Ananas comosus, bromelain A, gel filtration
19700
-
Ananas comosus, bromelain B, gel filtration
22500
-
Ananas comosus, gel filtration
23000
23430
-
Ananas comosus, enzyme form F9, mass spectroscopy
23550
-
isozyme SBA7A, mass spectroscopy
23560
-
isozyme SBA/b, mass spectroscopy
23800
-
SDS-PAGE
24400
-
Ananas comosus, enzyme form F4, mass spectroscopy
24470
-
Ananas comosus, enzyme form F5, mass spectroscopy
25600
-
x * 25600, Ananas comosus, enzyme forms SB1 and SB2, SDS-PAGE
26000
27000
-
x * 27000, Ananas comosus, enzyme forms SBB 1-3, SDS-PAGE
28000
28400
-
Ananas comosus, sedimentation velocity and equilibrium ultracentrifugation experiments
28500
-
1 * 28500, Ananas comosus, SDS-PAGE
33000
-
Ananas comosus, determination from sedimentation and diffusion coefficient
35000
-
x * 35000, Ananas comosus, bromelain A, SDS-PAGE
35300
-
Ananas comosus, sedimentation velocity measurements
36000
-
x * 36000, recombinant enzyme, SDS-PAGE
37000
-
Ananas comosus, gel filtration
43000
-
SDS-PAGE, recombinant protein
57000
-
x * 57000, His-tagged recombinant enzyme, SDS-PAGE
additional information