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Literature summary for 3.4.22.32 extracted from

  • Esti, M.; Benucci, I.; Liburdi, K.; Garzillo, A.M.
    Effect of wine inhibitors on free pineapple stem bromelain activity in a model wine system (2011), J. Agric. Food Chem., 59, 3391-3397.
    View publication on PubMed
Search for more literature for 3.4.22.32

Inhibitors

Inhibitors Comment Organism Structure
ethanol competitive inhibitor Ananas comosus
additional information gallic tannins or ellagic tannins show no inhibitory activity Ananas comosus
sulfur dioxide strong inhibitor Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, free SO2 25 mg/l Ananas comosus
0.052
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, seed 1.2 g/l Ananas comosus
0.056
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, free SO2 10 mg/l Ananas comosus
0.079
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, skin 0.99 g/l Ananas comosus
0.0952
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, seed 0.3 g/l Ananas comosus
0.118
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ethanol 0% Ananas comosus
0.152
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, skin 0.25 g/l Ananas comosus
0.22
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, gallic 0.02 g/l Ananas comosus
0.25
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ethanol 12% Ananas comosus
0.25
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, without free SO2 Ananas comosus
0.25
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, without gallic Ananas comosus
0.25
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, without skin Ananas comosus
0.2558
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ellagic 0.02 g/l Ananas comosus
0.27
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ellagic 0.04 g/l Ananas comosus
0.27
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, gallic 0.03 g/l Ananas comosus
0.305
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ethanol 18% Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bz-Phe-Val-Arg-4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?

Synonyms

Synonyms Comment Organism
stem bromelain
-
 Ananas comosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Ananas comosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.39
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, free SO2 25 mg/l Ananas comosus
0.96
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, free SO2 10 mg/l Ananas comosus
4.32
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, seed 1.2 g/l Ananas comosus
6.31
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, skin 0.99 g/l Ananas comosus
6.55
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, seed 0.3 g/l Ananas comosus
8.44
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, skin 0.25 g/l Ananas comosus
16.94
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, gallic 0.03 g/l Ananas comosus
18.33
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ellagic 0.04 g/l Ananas comosus
18.35
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, gallic 0.02 g/l Ananas comosus
19.23
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ellagic 0.02 g/l Ananas comosus
19.43
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ethanol 0% Ananas comosus
20.03
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ethanol 12% Ananas comosus
20.03
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, without free SO2 Ananas comosus
20.03
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, without gallic Ananas comosus
20.03
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, without skin Ananas comosus
21.45
-
 Bz-Phe-Val-Arg-4-nitroanilide pH 3.2, 25°C, ethanol 18% Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2
-
 assay at Ananas comosus