3.4.22.2: papain
This is an abbreviated version!
For detailed information about papain, go to the full flat file.
Word Map on EC 3.4.22.2
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3.4.22.2
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cathepsins
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proteinases
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chymotrypsin
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pepsin
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cystatins
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bromelain
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igg
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hydrolysates
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cartilage
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immunoglobulin
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ficin
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pronase
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proteoglycans
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erythrocyte
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glycosaminoglycans
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alpha-chymotrypsin
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elastase
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neuraminidase
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chondroitin
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alcalase
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subtilisin
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latex
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thermolysin
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carica
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allergen
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articular
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agglutination
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collagenase
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emphysema
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plasmin
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kininogens
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fab\'2
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anti-d
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intra-articular
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bacteriocins
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pineapple
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legumains
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l-like
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pancreatin
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meromyosin
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dermatan
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emphysematous
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chondroitinase
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antiglobulin
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nutrition
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actin-activated
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medicine
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food industry
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enzyme-treated
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industry
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alloantibody
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subfragment-1
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analysis
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synthesis
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carlsberg
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biotechnology
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procathepsins
- 3.4.22.2
- cathepsins
- proteinases
- chymotrypsin
- pepsin
- cystatins
- bromelain
- igg
- hydrolysates
- cartilage
- immunoglobulin
- ficin
- pronase
- proteoglycans
- erythrocyte
- glycosaminoglycans
- alpha-chymotrypsin
- elastase
- neuraminidase
- chondroitin
- alcalase
- subtilisin
- latex
- thermolysin
- carica
- allergen
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articular
-
agglutination
- collagenase
- emphysema
- plasmin
- kininogens
-
fab\'2
-
anti-d
-
intra-articular
-
bacteriocins
- pineapple
- legumains
-
l-like
- pancreatin
- meromyosin
- dermatan
-
emphysematous
- chondroitinase
-
antiglobulin
- nutrition
-
actin-activated
- medicine
- food industry
-
enzyme-treated
- industry
-
alloantibody
-
subfragment-1
- analysis
- synthesis
-
carlsberg
- biotechnology
-
procathepsins
Reaction
Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1' =
Synonyms
Adolph's Meat Tenderizer, arbuz, CpXCP5, EC 3.4.4.10, enzeco papain, papain, papain-like cysteine protease, papain-like protease, papaine, papaya peptidase I, papaya proteinase 1, Papaya proteinase I, papayotin, PLCP, PLpro, PPI, summetrin, velardon
ECTree
3.4.22.2 papainAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 3.4.22.2 - papain
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
22
25
40
50
55
56
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at pH 2.0, partially reduced papain, single cooperative transition with a midpoint at 55.61°C
60
65
70
75
80
90
additional information
22
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room temperature, pH 8.7, 95% loss of activity after 8 days, then the preparation maintains constant activity over an 86-day period, papain included in porous glass
22
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residual activity after 30 storage: 25% free papain, 80% immobilized papain
40
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single disulfide reduced carboxymethylated papain, at neutral pH, the protein started unfolding after 40°C
50
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oxidized, inactive enzyme, in absence of activator, 67% of the initial activity remains after 28 days
55
390 min, the soluble enzyme retains 41.9% of its activity, enzyme immobilized on hybrid nanoflowers retains 77.2% of its activity
60
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half-life of wild-type, mutant K174R/V32S and mutant K174R/V32S/G36S is 77, 114, and 171 min, respectively
65
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half-life of wild-type, mutant K174R/V32S and mutant K174R/V32S/G36S is 35, 45, and 80 min, respectively
70
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single disulfide reduced carboxymethylated papain, at neutral pH, midpoint of transition is 70.33°C
70
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30 min, the immobilized enzyme is completely stable, while the soluble enzyme loses activity
75
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2 h, free papain loses 60% of its activity, papain immobilized on the poly(glycidyl methacrylate-co-ethylene dimethylacrylate) monolith loses 10% of its activity
75
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the native enzyme loses nearly 50% of its activity after heating at 75°C for 40 min, and it retains only 20% activity after heating for 80 min
75
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immobilized enzyme loses 66% of its activity after 300 min incubation at 75°C
80
total activity losses for the cotton-immobilized unmodified papain and immobilized benzenetricarboxylic papain after 4 at pH 8.0
80
at 80°C, the enzyme immobilized on multi-walled carbon nanotubes retains more than 70% of the initial activity after 1 h incubation. A nearly complete inactivation of free papain is observed
80
half-life: 18 min (soluble enzyme). Immobilized papain retains 80% of its original activity after 1 h incubation
90
half-life: 6 min (soluble enzyme), 40 min (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide). Immobilized papain retains 45% of its original activity after 1 h incubation
90
the enzyme immobilized on multi-walled carbon nanotubes retains above 50% of the initial activity after 1 h incubation while the activity of the free enzyme is completely inactivated after 1 h incubation
additional information
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the enzyme is stable to heat in the oxidized, inactive form, activity is rapidly lost in presence of 2-mercaptopropionic acid, hippuric acid alters heat stability
additional information
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temperature and guanidine hydrochloride induced unfolding transitions of papain at pH 2.0 are biphasic, implying independent and sequential unfolding of its two domains. The N-domain unfolds initially
additional information
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thermal denaturation studies show that the binding of Ca2+ and Mg2+ brings about change in the thermal stability of papain at various concentrations of these metal ions. No significant change in the alpha-helix and beta-sheet structure of the papain upon binding of these metal ions
additional information
thermal unfolding of the prosegment, in acid medium the enzyme unfolds in a globule-like conformation, irreversible without intermediate states, overview
