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3.4.22.2: papain

This is an abbreviated version!
For detailed information about papain, go to the full flat file.

Word Map on EC 3.4.22.2

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1' =

Synonyms

Adolph's Meat Tenderizer, arbuz, CpXCP5, EC 3.4.4.10, enzeco papain, papain, papain-like cysteine protease, papain-like protease, papaine, papaya peptidase I, papaya proteinase 1, Papaya proteinase I, papayotin, PLCP, PLpro, PPI, summetrin, velardon

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.2 papain

Crystallization

Crystallization on EC 3.4.22.2 - papain

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a complex of chagasin, a protein inhibitor from Trypanosoma cruzi, and papain is crystallized by using the hanging-drop vapor diffusion method at 18°C, the high-resolution crystal structure shows an inhibitory wedge comprising three loops, which forms a number of contacts responsible for the high-affinity binding, the present chagasin-papain complex provides a reliable model of chagasin-cruzipain interactions
complex with benzyloxycarbonyl-Arg-Leu-Val-Gly-CHN2
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complex with E-64
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crystal structure of the complex of papain with recombinant human stefin B
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crystal structure of the papain-succinyl-Gln-Val-Val-Ala-Ala-p-nitroanilide complex at 1.7 -A resolution
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enzyme structure of the complex of papain with N-[N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucyl]-isoamylamide
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hanging drop vapour diffusion method with 50% ethanol, 0.01 M sodium acetate, crystallization of a papain-inhibitor of protease complex is not possible
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papain complexed with cathepsin B-specific covalent-type inhibitor
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papain-inhibitor complex with benzyloxycarbonyl-Leu-Leu-leucinal or benzyloxycarbonyl-L-Leu-L-Leu methoxymethyl ketone
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papain-leupeptin complex
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structure of papain complexed with benzyloxycarbonyl-L-Phe-L-Ala-L-Ala chloromethylketone
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tarocystatin-papain complex and C-terminal cystatin-like extension-papain complex, hanging drop vapor diffusion method, the tarocystatin-papain complex is crystallized from a drop containing 15% PEG monomethyl ether 2000, 0.05 M sodium acetate trihydrate, pH 4.6, 0.1 M ammonium sulfate against a reservoir of 30% PEG monomethyl ether 2000, 0.1 M sodium acetate trihydrate, pH 4.6, and 0.2 M ammonium sulfate. The complex of CtE-papain is crystallized from a drop containing 0.05 M HEPES, pH 7.5, and 35% (v/v) 2-methyl-2,4-pentanediol against a reservoir of 0.1 M HEPES, pH 7.5, and 70% (v/v) 2-methyl-2,4-pentanediol