3.4.22.2: papain
This is an abbreviated version!
For detailed information about papain, go to the full flat file.
Word Map on EC 3.4.22.2
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3.4.22.2
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cathepsins
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proteinases
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chymotrypsin
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pepsin
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cystatins
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bromelain
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igg
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hydrolysates
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cartilage
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immunoglobulin
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ficin
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pronase
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proteoglycans
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erythrocyte
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glycosaminoglycans
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alpha-chymotrypsin
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elastase
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neuraminidase
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chondroitin
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alcalase
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subtilisin
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latex
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thermolysin
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carica
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allergen
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articular
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agglutination
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collagenase
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emphysema
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plasmin
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kininogens
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fab\'2
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anti-d
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intra-articular
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bacteriocins
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pineapple
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legumains
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l-like
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pancreatin
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meromyosin
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dermatan
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emphysematous
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chondroitinase
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antiglobulin
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nutrition
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actin-activated
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medicine
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food industry
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enzyme-treated
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industry
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alloantibody
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subfragment-1
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analysis
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synthesis
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carlsberg
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biotechnology
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procathepsins
- 3.4.22.2
- cathepsins
- proteinases
- chymotrypsin
- pepsin
- cystatins
- bromelain
- igg
- hydrolysates
- cartilage
- immunoglobulin
- ficin
- pronase
- proteoglycans
- erythrocyte
- glycosaminoglycans
- alpha-chymotrypsin
- elastase
- neuraminidase
- chondroitin
- alcalase
- subtilisin
- latex
- thermolysin
- carica
- allergen
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articular
-
agglutination
- collagenase
- emphysema
- plasmin
- kininogens
-
fab\'2
-
anti-d
-
intra-articular
-
bacteriocins
- pineapple
- legumains
-
l-like
- pancreatin
- meromyosin
- dermatan
-
emphysematous
- chondroitinase
-
antiglobulin
- nutrition
-
actin-activated
- medicine
- food industry
-
enzyme-treated
- industry
-
alloantibody
-
subfragment-1
- analysis
- synthesis
-
carlsberg
- biotechnology
-
procathepsins
Reaction
Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1' =
Synonyms
Adolph's Meat Tenderizer, arbuz, CpXCP5, EC 3.4.4.10, enzeco papain, papain, papain-like cysteine protease, papain-like protease, papaine, papaya peptidase I, papaya proteinase 1, Papaya proteinase I, papayotin, PLCP, PLpro, PPI, summetrin, velardon
ECTree
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Organic Solvent Stability
Organic Solvent Stability on EC 3.4.22.2 - papain
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acetonitrile
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papain retains almost all its catalytic activity after 24 h of incubation in the presence of 99% (v/v) acetonitrile with a more compact conformation
dimethyl formamide
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papain shows a complete inactivation after 24 h when exposed to those media containing 90% (v/v) dimethyl formamide because of an irreversible conformational change
Glycerol
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in the presence of 20% (v/v) glycerol the enzyme retains nearly 80% of its activity even after heating at 75°C for 40 min and it retains almost 70% of its activity even after heating for 80 min, at 30% (v/v) glycerol the enzyme retains nearly 80 and 65% of its activity after heating for 40 and 80 min, respectively at 75°C
hexadecyltrimethyl ammonium bromide
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the presence of hexadecyltrimethyl ammonium bromide allows 41% recovery of enzymatic activity of acid-unfolded papain in the presence of hexadecyltrimethyl ammonium bromide the enzyme exists as a compact intermediate with regain of native-like secondary and partial tertiary structure as well as high 8-anilino-1-naphthalene-sulfonic acid binding with the partially recovered enzymatic activity
Methanol
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papain shows 80% loss of activity after 24 h incubation in 90% (v/v) methanol although no global conformational change and minor secondary structure rearrangements are detected
SDS
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the presence of SDS allows 43% recovery of enzymatic activity of acid-unfolded papain, addition of 8 mM SDS results in the loss of 8-anilino-1-naphthalene-sulfonic acid binding sites exhibited by a decrease in 8-anilino-1-naphthalene-sulfonic acid fluorescence intensity, suggesting the burial of hydrophobic patches, papain at low pH and in the presence of SDS exists in a partially folded state characterized by native-like secondary structure and tertiary folds
tetrahydrofuran
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30%, inactivation within 30 min. Sugars protect papain from tetrahydrofuran-induced inactivation in the decreasing order D-ribose, D-fructose, D-glucose, D-saccharose, D-raffinose. D-ribose at 1.6 mol per l is the most effective stabiliser. In 60% tetrahydrofuran in the presence of ribose, papain preserves about 55% of its initial activity after 2 h
Tween
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the presence of Tween-20 allows 39% recovery of enzymatic activity of acid-unfolded papain in the presence of Tween-20, acid-unfolded papain exists as a compact intermediate with molten-globule-like characteristics