3.4.22.2: papain
This is an abbreviated version!
For detailed information about papain, go to the full flat file.
Word Map on EC 3.4.22.2
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3.4.22.2
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cathepsins
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proteinases
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chymotrypsin
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pepsin
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cystatins
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bromelain
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igg
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hydrolysates
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cartilage
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immunoglobulin
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ficin
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pronase
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proteoglycans
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erythrocyte
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glycosaminoglycans
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alpha-chymotrypsin
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elastase
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neuraminidase
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chondroitin
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alcalase
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subtilisin
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latex
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thermolysin
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carica
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allergen
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articular
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agglutination
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collagenase
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emphysema
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plasmin
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kininogens
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fab\'2
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anti-d
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intra-articular
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bacteriocins
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pineapple
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legumains
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l-like
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pancreatin
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meromyosin
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dermatan
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emphysematous
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chondroitinase
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antiglobulin
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nutrition
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actin-activated
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medicine
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food industry
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enzyme-treated
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industry
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alloantibody
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subfragment-1
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analysis
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synthesis
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carlsberg
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biotechnology
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procathepsins
- 3.4.22.2
- cathepsins
- proteinases
- chymotrypsin
- pepsin
- cystatins
- bromelain
- igg
- hydrolysates
- cartilage
- immunoglobulin
- ficin
- pronase
- proteoglycans
- erythrocyte
- glycosaminoglycans
- alpha-chymotrypsin
- elastase
- neuraminidase
- chondroitin
- alcalase
- subtilisin
- latex
- thermolysin
- carica
- allergen
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articular
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agglutination
- collagenase
- emphysema
- plasmin
- kininogens
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fab\'2
-
anti-d
-
intra-articular
-
bacteriocins
- pineapple
- legumains
-
l-like
- pancreatin
- meromyosin
- dermatan
-
emphysematous
- chondroitinase
-
antiglobulin
- nutrition
-
actin-activated
- medicine
- food industry
-
enzyme-treated
- industry
-
alloantibody
-
subfragment-1
- analysis
- synthesis
-
carlsberg
- biotechnology
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procathepsins
Reaction
Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1' =
Synonyms
Adolph's Meat Tenderizer, arbuz, CpXCP5, EC 3.4.4.10, enzeco papain, papain, papain-like cysteine protease, papain-like protease, papaine, papaya peptidase I, papaya proteinase 1, Papaya proteinase I, papayotin, PLCP, PLpro, PPI, summetrin, velardon
ECTree
3.4.22.2 papainAdvanced search results
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results (Turnover Number
Turnover Number on EC 3.4.22.2 - papain
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
180
benzyl-Phe-Val-Arg-4-nitroanilide
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McIlvaine buffer, pH 3.2, 25°C, papain from ripe fruit
189
benzyl-Phe-Val-Arg-4-nitroanilide
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McIlvaine buffer, pH 3.2, 25°C, papain from papaya latex
215
benzyl-Phe-Val-Arg-4-nitroanilide
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McIlvaine buffer + 12% ethanol, pH 3.2, 25°C, papain from papaya latex
217
benzyl-Phe-Val-Arg-4-nitroanilide
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tartaric buffer containing 12% ethanol, pH 3.2, 25°C, papain from papaya latex
290
benzyl-Phe-Val-Arg-4-nitroanilide
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McIlvaine buffer + 12% ethanol, pH 3.2, 25°C, papain from ripe fruit
309
benzyl-Phe-Val-Arg-4-nitroanilide
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tartaric buffer containing 12% ethanol, pH 3.2, 25°C, papain from ripe fruit
0.79
benzyloxycarbonyl-Arg-Arg 4-methylcoumarin-7-amide
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mutant enzyme V133A/V157G/S205E
9.3
benzyloxycarbonyl-Arg-Arg 4-methylcoumarin-7-amide
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mutant enzyme V133A/S205E
0.42
benzyloxycarbonyl-citrullyl-Arg 4-methylcoumarin-7-amide
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mutant enzyme V133A/V157G/S205E
5.67
benzyloxycarbonyl-citrullyl-Arg 4-methylcoumarin-7-amide
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mutant enzyme V133A/S205E
8.14
benzyloxycarbonyl-citrullyl-Arg 4-methylcoumarin-7-amide
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mutant enzyme V133A/V157G/S205E
51
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
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recombinant wild-type enzyme
52
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
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commercial enzyme preparation
2.78
benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide
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mutant enzyme V133A/V157G/S205E
16.9
benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide
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mutant enzyme V133A/S205E
0.00000035
casein
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papain immobilized on sepharose 6B in presence of 200 mM cysteine, pH 7.5, 22°C
0.0102
casein
enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide, pH 7.5, 23°C
1.386
casein
cotton-immobilized papain modified by pyromellitic anhydride, pH 8.0, 45°C
1.5
casein
enzyme immobilized on multi-walled carbon nanotubes, pH 7.5, 23°C
1.604
casein
cotton-immobilized papain modified by 1,2,4-benzenetricarboxylic anhydride, pH 8.0, 45°C
0.03 - 0.55
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans
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pH 6.8, 40°C, in presence of 5% methanol
0.41
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans
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pH 6.8, 40°C, in presence of 0.6% dimethylformamide
2.77
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans
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pH 6.8, 40°C, in presence of 1% methanol
3 - 6
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans
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pH 6.8, 40°C, in presence of 1% methanol
6.86
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans
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pH 6.8, 40°C, in presence of 5% methanol
475
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2
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at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine
1773
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2
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at pH 7.4, in 100 mM phosphate-buffered saline
243
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2
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at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine
1503
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2
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at pH 7.4, in 100 mM phosphate-buffered saline
0.79
ubiquitin-7-amido-4-trifluoromethylcoumarin
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pH not specified in the publication, 30°C, PLpro(1541-1858, DNA fragment)
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0.96
ubiquitin-7-amido-4-trifluoromethylcoumarin
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pH not specified in the publication, 30°C, PLpro(1600-1858, DNA fragment)
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additional information
additional information
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kcat (casein) for immobilized papain is much lower than for free papain
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