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3.4.22.2: papain

This is an abbreviated version!
For detailed information about papain, go to the full flat file.

Word Map on EC 3.4.22.2

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1' =

Synonyms

Adolph's Meat Tenderizer, arbuz, CpXCP5, EC 3.4.4.10, enzeco papain, papain, papain-like cysteine protease, papain-like protease, papaine, papaya peptidase I, papaya proteinase 1, Papaya proteinase I, papayotin, PLCP, PLpro, PPI, summetrin, velardon

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.2 papain

Renatured

Renatured on EC 3.4.22.2 - papain

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
acid-unfolded papain in the presence of 8 mM SDS and 3.5 mM hexadecyltrimethyl ammonium bromide retains its partial tertiary structure
-
fractions containing propapain are pooled and refolded by rapid dilution into a refolding buffer at pH 8.6, the denatured protein folds best at pH 8.5 and at 4°C, while pH values above 8.8 and below 7.0 are not suitable for folding, folding process requires addition of 0.5 M arginine, 1 mM glutathione, 0.1 mM GSSG and 15% glycerol
recombinant papain prosegment from Escherichia coli strain BL21(DE3) inclusion bodies by 2% Triton X-100, 8 M guanidinium hydrochloride, and dialysis against 50 mM phosphoric acid/NaOH buffer, pH 7.0, and centrifugation at 45000 rpm for 1 h