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3.4.21.53: Endopeptidase La

This is an abbreviated version!
For detailed information about Endopeptidase La, go to the full flat file.

Word Map on EC 3.4.21.53

Reaction

hydrolysis of proteins in presence of ATP =

Synonyms

AAA+ Lon protease, AAA+ protease, AAAP, AF0364, AfLon, archaeal Lon protease, ATP-dependent lon protease, ATP-dependent Lon proteinase, ATP-dependent PIM1 protease, ATP-dependent protease La, ATP-dependent protease lon, ATP-dependent protease LonA, ATP-dependent serine proteinase, ATP-independent Lon-like protease, bacterial protease lon, BPP1347, ClpXP, EcLon, Ec-Lon, Ec-Lon protease, EcLon, EcLon protease, ELon, Escherichia coli proteinase La, Escherichia coli serine proteinase La, Gene lon protease, Gene lon proteins, hLon, human ATP-dependent protease, human lon protease, HVO_0783, i-AAA Protease, la, La protease, lon, lon (la) protease, lon (Pim1p) protease, Lon AAA+ protease, lon ATP-dependent protease, lon protease, LON protease 1, Lon protein, Lon proteinase, lon-like protease, Lon-like-Ms, lon1, lon2, lon3, lon4, lonA, lonB, lonB protease, LonC, LonC protease, lonD, LONP1, lonR9, LONRF1, lonS, lonTK, lonV, mitochondrial ATP-dependent protease, mitochondrial ATP-dependent protease La, mitochondrial Lon protease, MLon, Ms-Lon, Msm 1754, Msm_1754, MtaLonA, MtaLonC, Nmag_2822, NmLon, non-canonical RNA viral Lon proteinase, peroxisomal Lon protease, PIM1, PIM1 protease, PIM1 proteinase, Pim1p, PLon, protease, Protease La, protease lon, Proteinase La, Proteinase, Escherichia coli serine, La, Proteinase, La, Proteins, gene lon, Proteins, specific or class, gene lon, ScLon, serine protease, Serine protease La, Ta1081, Thela2p4_005149, Thela2p4_006664, TK1264, TonLonB, TON_0529, yeast mitochondrial lon, yeast protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.53 Endopeptidase La

Purification

Purification on EC 3.4.21.53 - Endopeptidase La

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
139fold, ion exchange chromatography and gel filtration
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active lon carrying six C-terminal histidine residues is overexpressed in yeast, is rapidly purified to homogeneity from an isolated mitochondrial extract on a Ni21-NTA column, and purified to homogeneity by gel filtration on a Superose 6 column
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by gel filtration, to near homogeneity
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by successive Ni2+-NTA affinity chromatography and anion-exchange chromatography
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gel filtration
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human lon is overproduced and purified from the Rosetta Escherichia coli strain. Human mitochondrial lon degrades mouse StAR in the presence of ATP, but not in its absence
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lon-N119 purified. Digestion of lonA by alpha-chymotrypsin yields a stable fragment consisting of residues 491-584 purified. lon proteolytic domain purified
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lonR9 product
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mutants purified to homogeneity
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on Ni2+-NTA column
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overproduced and purified from isolated yeast mitochondria
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protein purification procedure utilizes mitochondria from the mutant strain pep949 (MATa/alpha, prl 1-1122, prc 1-40Z, pep 4-3, leu2, trp 1, ura 3-52)
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proteolytically inactive mutant Lon-S679A
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recombinant C-terminally His6-tagged wild-type and mutant enzyme from Escherichia coli by nickel affinity chromatography and gel filtation
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recombinant C-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant enzyme
recombinant enzyme from Escherichia coli strain Rosetta (DE3) by ultrafiltration and gel filtration
recombinant His-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme mutant from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by ultracentrifugation, nickel affinity chromatography and gel filtration
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recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain CH1019 by nickel affinity chromatography
recombinant His6-tagged enzyme by affinity chromatography
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recombinant His6-tagged enzyme, solubilized from Escherichia coli strain BL21(DE3) Codon Plus (RIL) inclusion bodies, by nickel affinity chromatography
recombinant membrane enzyme in Escherichia coli
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recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography and gel filtration
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recombinant N-terminally His6-tagged wild-type and mutant S684A Lon from Escherichia coli strain BLR-DE3 by nickel affinity chromatography
recombinant N-terminally truncated ClpX lacking residues 1-62, from Escherichia coli strain BL21(DE3)
recombinant protein
recombinant selenomethionine-labeled N-terminally His6-tagged N-terminal domain of mutant L91M/L188M/I359M enzyme from Escherichia coli strain B834(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, anion exchange chromatography, and gel filtration
recombinant truncated His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, the enzyme is reduced to the AAA+ domain by proteolytic treatment with trypsin followed by gel filtration
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recombinant wild-type and mutant enzymes from Escherichia coli strain W3110