Cloned (Comment) | Organism |
---|---|
gene lon | Escherichia coli |
gene LONP1, recombinant expression of the enzyme in Escherichia coli strain Rosetta (DE3) | Homo sapiens |
General Stability | Organism |
---|---|
ELon ist quite stable against digestion by trypsin, ADP binding does not protect. ELon binds to ADP and undergoes at least one structural change that exposes a tryptic digestion site | Escherichia coli |
hLon ist rapidly digested by trypsin, ADP binding does not protect | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | non-competitive inhibition, ELon binds to ADP and undergoes at least one structural change that exposes a tryptic digestion site | Escherichia coli | |
ADP | non-competitive inhibition dependent on substrate concentration, steady-state ADP inhibition study, overview. hLon binds to ADP and undergoes at least one structural change that exposes a tryptic digestion site | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.027 | - |
FRETN 89-98 | pH 8.0, 37°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Escherichia coli | 5829 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9M0 | - |
- |
Homo sapiens | P36776 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain Rosetta (DE3) by ultrafiltration and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FRETN 89-98 + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | Lon protease has three activities: intrinsic ATPase, substrate-stimulated ATPase, and ATP-dependent proteolysis. Lon preferentially degrades damaged or misfolded proteins at its proteolytic site while the ATP is bound and hydrolyzed into ADP and phosphate at its ATPase site | Homo sapiens | ? | - |
? | |
additional information | Lon protease has three activities: intrinsic ATPase, substrate-stimulated ATPase, and ATP-dependent proteolysis. Lon preferentially degrades damaged or misfolded proteins at its proteolytic site while the ATP is bound and hydrolyzed into ADP and phosphate at its ATPase site | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 83000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent protease La | - |
Escherichia coli |
ELon | - |
Escherichia coli |
hLon | - |
Homo sapiens |
lon | - |
Homo sapiens |
lon | - |
Escherichia coli |
lon protease | - |
Homo sapiens |
lon protease | - |
Escherichia coli |
LONP1 | - |
Homo sapiens |
mitochondrial ATP-dependent protease La | - |
Homo sapiens |
Protease La | - |
Homo sapiens |
Protease La | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.63 | - |
FRETN 89-98 | pH 8.0, 37°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, ADP inhibition study | Homo sapiens | |
1.79 | - |
ADP | pH 8.0, 37°C, recombinant enzyme | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | human enzyme hLon and Escherichia coli enzyme ELon bind to ADP and undergo at least one structural change that exposes the same tryptic digestion site, suggesting the presence of at least one conserved structural change in the two enzyme homologues upon binding to ADP | Homo sapiens |
evolution | human enzyme hLon and Escherichia coli enzyme ELon bind to ADP and undergo at least one structural change that exposes the same tryptic digestion site, suggesting the presence of at least one conserved structural change in the two enzyme homologues upon binding to ADP | Escherichia coli |
physiological function | in eukaryotes, Lon 1 is localized in the mitochondria and helps maintain proper cellular function. In humans, Lon is critical for maintaining the structure and integrity of mitochondria and has been found to selectively degrade accumulating proteins damaged by oxidative stress over their native counterparts | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.46 | - |
FRETN 89-98 | pH 8.0, 37°C, recombinant enzyme | Homo sapiens |