3.4.21.53: Endopeptidase La
This is an abbreviated version!
For detailed information about Endopeptidase La, go to the full flat file.
Reaction
hydrolysis of proteins in presence of ATP
=
Synonyms
AAA+ Lon protease, AAA+ protease, AAAP, AF0364, AfLon, archaeal Lon protease, ATP-dependent lon protease, ATP-dependent Lon proteinase, ATP-dependent PIM1 protease, ATP-dependent protease La, ATP-dependent protease lon, ATP-dependent protease LonA, ATP-dependent serine proteinase, ATP-independent Lon-like protease, bacterial protease lon, BPP1347, ClpXP, EcLon, Ec-Lon, Ec-Lon protease, EcLon, EcLon protease, ELon, Escherichia coli proteinase La, Escherichia coli serine proteinase La, Gene lon protease, Gene lon proteins, hLon, human ATP-dependent protease, human lon protease, HVO_0783, i-AAA Protease, la, La protease, lon, lon (la) protease, lon (Pim1p) protease, Lon AAA+ protease, lon ATP-dependent protease, lon protease, LON protease 1, Lon protein, Lon proteinase, lon-like protease, Lon-like-Ms, lon1, lon2, lon3, lon4, lonA, lonB, lonB protease, LonC, LonC protease, lonD, LONP1, lonR9, LONRF1, lonS, lonTK, lonV, mitochondrial ATP-dependent protease, mitochondrial ATP-dependent protease La, mitochondrial Lon protease, MLon, Ms-Lon, Msm 1754, Msm_1754, MtaLonA, MtaLonC, Nmag_2822, NmLon, non-canonical RNA viral Lon proteinase, peroxisomal Lon protease, PIM1, PIM1 protease, PIM1 proteinase, Pim1p, PLon, protease, Protease La, protease lon, Proteinase La, Proteinase, Escherichia coli serine, La, Proteinase, La, Proteins, gene lon, Proteins, specific or class, gene lon, ScLon, serine protease, Serine protease La, Ta1081, Thela2p4_005149, Thela2p4_006664, TK1264, TonLonB, TON_0529, yeast mitochondrial lon, yeast protease
ECTree
Cloned
Cloned on EC 3.4.21.53 - Endopeptidase La
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
C-terminally His6-tagged lon expressed in Escherichia coli strain CH1019 carrying lon expression plasmid pET21b-LonH6
-
cDNA clone pHHCPK77, 51 kDa fragment expressed in Escherichia coli BL21(DE3)
cDNAcloned and expressed in Escherichia coli Rosetta strain
-
cloned and expressed in Escherichia coli MC1061
-
cloned and expressed in Saccharomyces cerevisiae, Lon protease substitutes for PIM1 protease
-
cloning of the P-domain, expression as MBP fusion protein in Escherichia coli BL21 (DE3)
-
cloning the lon gene in pGEX-KG
-
cloning, sequencing and disruption of PIM1, the nuclear gene encoding mitochondrial ATP-dependent protease
-
DNA sequence analysis, expression analysis
-
DNA sequencing and analysis
-
expression as green fluorescent protein-fusion protein
-
expression in Escherichia coli of intein fusion protein that consists of an N-terminal poly-His tag followed by Escherichia coli MBP, the 23 C-terminal residues of the N-extein, the 333 residues of the lon protease intein, the 27 N-terminal residues of the C-extein, and a Cterminal GST
-
expression in Schneider cell
-
expression of a deletion mutant lacking the putative membrane-anchoring region, residues 134-170
expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta 2 (DE3)
-
expression of the proteolytic domain, residues 741-959, in Escherichia coli
gene lon, domain organization, overview
gene lon, expression analysis in wild-type and mutant strains by quantitative, real-time reverse transcription PCR, overview
gene lon, functional recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain CH1019
gene lon, functional recombinant expression of wild-type and mutant enzymes in Escherichia coli strain W3110
gene lon, recombinant expression of C-terminally His6-tagged wild-type and mutant enzyme in Escherichia coli strain BL21 (DE3), expression of selenomethionine-labeled enzymes in Escherichia coli strain B834 (DE3)
-
gene lon, recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene lon, recombinant expression of His-tagged enzyme mutant in Escherichia coli
gene lon, recombinant expression of N-terminally truncated ClpX lacking residues 1-62, i.e. ClpXDELTAN, in Escherichia coli strain BL21(DE3)
gene lonA1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene lonC, cloning and expression of N-terminally His6-tagged residues 35-387 of the N-terminal domain of the mutant L91M/L188M/I359M enzyme with a TEV protease-cleavage site as selenomethionine-labeled protein in Escherichia coli strain B834(DE3)
gene LONP1, recombinant expression of GFP-tagged Lon in Saccharomyces cerevisiae strain BY4742 in mitochondrial nucleoids
gene LONP1, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Codon Plus (RIL) in inclusion bodies
gene LONP1, recombinant expression of the enzyme in Escherichia coli strain Rosetta (DE3)
gene Msm_1754, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene PFL_3985, functional recombinant expression of His-tagged enzyme in Escherichia coli
into the NdeI and BamHI sites of proEX, lon (proEX-Lon-His) expressed in Escherichia coli. Plasmid lon O/Ex expressed in COS cells
-
into vector pCR2.1 resulting in plasmids pLonAB and pLonCD
-
Lon cDNA cloned and expressed in yeast strain PIM1, complementing a LON deletion, also overexpressed in COS-7 cells
-
lonR9 cloned from Escherichia coli RGC123 and sequenced
-
LonTK cloned and expressed in Escherichia coli
mutants cloned into vector pET24a(+), expressed in Escherichia coli Rosetta (DE3) pLysS
mutants via plasmids pJW028 and pJW015 expressed in Escherichia coli BL21
-
N-terminal residues 1-245, expression in Escherichia coli
NdeI-BamHI fragment from proEx-1/lon cloned into pET24c(+), overexpressed in Escherichia coli Rosetta (DE3)
-
NPS3121 lon gene under the native promoter (210 bases upstream of the ORF) PCR amplified, cloned into the pBS-HA vector, NPS3121 lon gene injected into fully expended primary leaves of bean plants. DC3000 vacuum infiltrated into tomato plants
-
ORF of the lon gene PA1803 PCR-amplified and cloned into vector TopoBlunt. HindIII-XbaI fragment containing the lon gene ligated into vector pBBR1-MCS5, transformed into Escherichia coli XL-1 Blue and subsequently into Escherichia coli S17-1 for mobilization into Pseudomonas aeruginosa 74-D9 through conjugation
-
PCR fragment cloned into expression vector pQE30 and transformed into the lon-deficient mutant
-
PCR product cloned into the NcoI and HindIII sites of pET24d(+), overexpressed in Escherichia coli BL21 (DE3)
-
providing of the lon protease in trans via plasmid pBBRlon, which carries the cloned genomic locus harbouring the lon protease gene expressed from its own promoter
recombinant expression of His-tagged enzyme with deletion of a membrane-anchoring region (residues 134-170) in Escherichia coli strain BL21(DE3)
-
recombinant expression of His-tagged wild-type and mutant enzyme in Escherichia coli
-
recombinant expression of His6-tagged enzyme
-
recombinant expression of N-terminally His6-tagged wild-type and mutant S684A Lon in Escherichia coli strain BLR-DE3
recombinant lon expressed in COS-7 cells. HEK293 cell lines stably expressing wild-type lon and its internal deletion mutant delta75-490, by using the retrovirus expression system
-
recombinant wild-type and mutant lon overexpressed in Escherichia coli BL21 (DE3), using plasmids pHF020 and pHF031, respectively
-
truncated version of lon4 cloned into pCR T7/NT-TOPO and expressed in Escherichia coli strain BL21(DE3). GFP fused to the putative N-terminal targeting regions from lon1 or lon4 (ca. 80 amino acids) expressed in Arabidopsis protoplasts or in different tobacco suspension cultured cell lines
-
wild-type and mutant enzymes cloned and expressed in Escherichia coli BL21
-
wild-type and mutant PIM1 protease cloned into a centromere-based yeast shuttle vector
-
wild-type and S675A mutant expressed in Escherichia coli SGR9
-
-
-
gene Lonp1
-
gene LONP1, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Codon Plus (RIL) in inclusion bodies
gene LONP1, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Codon Plus (RIL) in inclusion bodies
3.4.21.53 Endopeptidase La
results (
results (
top
