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3.2.1.83: kappa-carrageenase

This is an abbreviated version!
For detailed information about kappa-carrageenase, go to the full flat file.

Word Map on EC 3.2.1.83

Reaction

4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl- =

Synonyms

Car19, carrageenase, kappa-, CGK, Cgk-K142, Cgk16A, CgkA, CgkAGH16, CgkS, CgkZ, kappa-car, kappa-carrageenanase, kappa-carrageenase, kappa-carrageenase-like protein

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.83 kappa-carrageenase

Temperature Stability

Temperature Stability on EC 3.2.1.83 - kappa-carrageenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 50
-
the purified kappa-carrageenase is stable in the range of 0-30°C and the residual enzyme activity is still retained at 96.1% of the control after treatment at 30°C for 1 h. The enzyme is inactivated rapidly at temperatures higher than 30°C and is almost inactivated at 50°C within 1 h
10 - 40
20
-
40% loss of activity within 5 days, complete loss of activity within 12 days
25
42% of residual activity is retained after 24 h at 25°C
28 - 75
-
the enzyme is stable at 28°C, but 95% of the activity is lost at 50-75°C for 30 min
37
-
5 days, 50% loss of activity
4
-
the half-life of free enzyme and enzyme immobilized on magnetic iron oxide nanoparticles at 4°C are 17.4 and 20.6 d, respectively
40 - 45
-
the enzyme retains more than 95% and 60% of the maximum enzymatic activity after 1 h of incubation at 40°C and below 45°C, respectively
40 - 50
-
over 80% of the total activity is retained after 60 min at 40°C. When the incubation temperature rises above 45°C, the activity of the enzyme decreases rapidly. Furthermore, the enzyme is almost inactivated at 50°C within 60 min
42 - 50
after incubation at 45°C for 4 h, approximately 50% of the enzymatic activity is retained. When the recombinant enzyme is incubated at 42.5°C for 4 h, more than 90% of the enzymatic activity is retained. The recombinant enzyme is stable below 42.5°C. Approximately 50% of the enzymatic activity is lost after 1 h of incubation at 47.5°C and after 30 min of incubation at 50°C
45
purified recombinant enzyme, pH 6.0, 15 min, 20% activity remaining
45 - 60
-
enzyme activity is stable (more than 91%) for 2 h at temperatures of above 45°C. However, 90% of the activity is lost after incubation at 50-60°C for 30 min
5
Pseudomonas carrageenovora
-
stable for 2 weeks
50 - 70
-
the enzyme retains more than 90% of its initial activity after incubation at 50-60°C for 24 h. The activity declines sharply after 60 min of incubation at 70°C
55
-
10 min, complete inactivation
60
-
purified enzyme, pH 7.0, inactivation within 60 min
70
-
purified enzyme, pH 7.0, inactivation within 30 min
additional information