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3.2.1.83: kappa-carrageenase

This is an abbreviated version!
For detailed information about kappa-carrageenase, go to the full flat file.

Word Map on EC 3.2.1.83

Reaction

4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl- =

Synonyms

Car19, carrageenase, kappa-, CGK, Cgk-K142, Cgk16A, CgkA, CgkAGH16, CgkS, CgkZ, kappa-car, kappa-carrageenanase, kappa-carrageenase, kappa-carrageenase-like protein

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.83 kappa-carrageenase

Purification

Purification on EC 3.2.1.83 - kappa-carrageenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, CM-cellulose 52 column chromatography, and Sephadex G-200 gel filtration
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ammonium sulfate precipitation, Sephadex 200 gel filtration, and Sephadex G-75 gel filtration
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ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DE-cellulose 52 anion-exchange chromatography
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by metal-affinity chromatography followed by cation exchange chromatography
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CM-Sepharose column chromatography
DEAE Sepharose column chromatography and HiTrap column chromatography
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His Trap column chromatography and Sephacryl S-100 gel filtration
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HisTrap column chromatography
native enzyme from culture supernatant by ammonium sulfate fractionation and adsorption chromatography
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native extracellular enzyme to electrophoretic homogeneity from culture supernatant by ammonium sulfate fractionation, dialysis, and two different steps of gel filtration
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Ni affinity column chromatography and Sephadex gel filtration
Ni-NTA Sepharose column chromatography
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Ni-Sepharose column chromatography
Q-Sepharose column chromatography and Sephadex G200 gel filtration
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recombinant CBM-tagged chimeric fusion enzyme cCgkA-cCglA by cellulose affinity purification using cellulose as a support or by nickel affinity chromatography
recombinant enzyme expressed in Escherichia coli
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recombinant extracelluar enzyme 388.6fold from Escherichia coli strain DH5alpha by ammonium sulfate fractionation, dialysis, anion and cation exchange chromatography to homogeneity
recombinant extracellular (24fold) and intracellular (19fold) His-tagged enzyme from Escherichia coli strain BL21 (DE3) cell-free extract and culture supernatant by ammonium sulfate fractionation, dialysis, nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from the periplasm of Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography
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