3.2.1.83: kappa-carrageenase
This is an abbreviated version!
For detailed information about kappa-carrageenase, go to the full flat file.
Word Map on EC 3.2.1.83
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3.2.1.83
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carrageenovora
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pseudoalteromonas
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carrageenases
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cd19-specific
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piggybac
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anti-cd19
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cd19-targeting
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tisagenlecleucel
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galactans
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alteromonas
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beta-agarase
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pharmacology
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molecular biology
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analysis
- 3.2.1.83
- carrageenovora
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pseudoalteromonas
-
carrageenases
-
cd19-specific
-
piggybac
-
anti-cd19
-
cd19-targeting
-
tisagenlecleucel
- galactans
- alteromonas
- beta-agarase
- pharmacology
- molecular biology
- analysis
Reaction
4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl- =
Synonyms
Car19, carrageenase, kappa-, CGK, Cgk-K142, Cgk16A, CgkA, CgkAGH16, CgkS, CgkZ, kappa-car, kappa-carrageenanase, kappa-carrageenase, kappa-carrageenase-like protein
ECTree
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Purification
Purification on EC 3.2.1.83 - kappa-carrageenase
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ammonium sulfate precipitation, CM-cellulose 52 column chromatography, and Sephadex G-200 gel filtration
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ammonium sulfate precipitation, Sephadex 200 gel filtration, and Sephadex G-75 gel filtration
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ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DE-cellulose 52 anion-exchange chromatography
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by metal-affinity chromatography followed by cation exchange chromatography
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DEAE Sepharose column chromatography and HiTrap column chromatography
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His Trap column chromatography and Sephacryl S-100 gel filtration
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native enzyme from culture supernatant by ammonium sulfate fractionation and adsorption chromatography
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native extracellular enzyme to electrophoretic homogeneity from culture supernatant by ammonium sulfate fractionation, dialysis, and two different steps of gel filtration
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Ni affinity column chromatography and Sephadex gel filtration
Ni-Sepharose column chromatography
recombinant CBM-tagged chimeric fusion enzyme cCgkA-cCglA by cellulose affinity purification using cellulose as a support or by nickel affinity chromatography
recombinant extracelluar enzyme 388.6fold from Escherichia coli strain DH5alpha by ammonium sulfate fractionation, dialysis, anion and cation exchange chromatography to homogeneity
recombinant extracellular (24fold) and intracellular (19fold) His-tagged enzyme from Escherichia coli strain BL21 (DE3) cell-free extract and culture supernatant by ammonium sulfate fractionation, dialysis, nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from the periplasm of Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography
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Ni affinity column chromatography and Sephadex gel filtration