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3.2.1.83: kappa-carrageenase

This is an abbreviated version!
For detailed information about kappa-carrageenase, go to the full flat file.

Word Map on EC 3.2.1.83

Reaction

4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-alpha-D-galactopyranosyl- =

Synonyms

Car19, carrageenase, kappa-, CGK, Cgk-K142, Cgk16A, CgkA, CgkAGH16, CgkS, CgkZ, kappa-car, kappa-carrageenanase, kappa-carrageenase, kappa-carrageenase-like protein

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.83 kappa-carrageenase

Cloned

Cloned on EC 3.2.1.83 - kappa-carrageenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain DH5alpha as extracellular enzyme
expressed in Brevibacillus choshinensis
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C43(DE3) cells
expressed in Pichia pastoris GS-115 cells
expression in Escherichia coli
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gene cgkA, recombinant expression of functional CBM-tagged chimeric fusion enzyme cCgkA-cCglA, consisting of the catalytic domains of kappa-carrageenase CgkA and lambda-carrageenase CglA from Pseudoalteromonas carrageenovora fused with a dockerin domain, in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain DH5alpha
gene cgkZ, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His-tagged enzyme with and without signal peptide extracellulary and intracellularly in Escherichia coli strain BL21 (DE3), cloning in Escherichia coli strain DH5alpha
gene cgkZ, recombinant expression of N-terminally His6-tagged extracellular enzyme in Escherichia coli strain BL21(DE3), 51% of the total kappa-carrageenase is secreted into culture medium, and 33% accumulates in the periplasmic space. Low temperature of 23°C and optimal isopropyl-beta-L-thiogalactoside concentration of 0.9 mM favor soluble protein expression, additives such as lactose, glycine, Tween-80, and Triton X-100 promote the release of intracellular enzymes, method optimization and evaluation, overview
His-tagged enzyme expression in the periplasm of Escherichia coli strain BL21(DE3)
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overexpressed as a His-tagged fusion protein in Escherichia coli
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