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1,10-phenanthroline
-
63.3% residual activity at 5 mM
AgNO3
-
1 mM, complete inhibition
Al3+
72.3% residual activity at 1 mM
AlCl3
-
1 mM, 39.7% inhibition
BaCl2
-
1 mM, 26.8% inhibition
CaCl2
-
1 mM, 50.7% inhibition
CuSO4
-
1 mM, 31.3% inhibition
FeCl3
-
1 mM, 36.5% inhibition
iodoacetic acid
-
79.7% residual activity at 5 mM
NaI
-
strong inhibition at 200 mM, inhibition is caused by iodide binding
phenylmethylsulfonyl fluoride
Sr2+
-
about 50% residual activity at 2 mM
Tris
-
strong inhibition at 200 mM
ZnCl2
-
1 mM, 50% inhibition
Ba2+
-
the enzyme activity is inhibited by 1 mM Ba2+
Ba2+
-
53.25% residual activity at 1 mM
Ba2+
-
98% residual activity at 1 mM
Ca2+
-
80% residual activity at 1 mM
Ca2+
-
the enzyme activity is inhibited by 1 mM Ca2+
Ca2+
-
96.9% residual activity at 10 mM
Ca2+
-
70.84% residual activity at 1 mM
Cd2+
-
about 80% residual activity at 2 mM
Cd2+
-
the enzyme activity is inhibited by 1 mM Cd2+
Co2+
-
13.2% residual activity at 10 mM
Co2+
-
almost complete inhibition at 1 mM
Cu2+
-
48.3% residual activity at 10 mM
Cu2+
-
2.1% residual activity at 50 mM
Cu2+
-
almost complete inhibition at 1 mM
Cu2+
26.3% residual activity at 1 mM
Cu2+
-
9.67% residual activity at 1 mM
Cu2+
-
32% residual activity at 1 mM
Cu2+
20.2% residual activity at 5 mM
EDTA
-
about 90% residual activity at 2 mM
EDTA
30.65% residual activity at 1 mM
EDTA
-
86% residual activity at 1 mM
EDTA
-
50.4% residual activity at 5 mM
EDTA
-
97.4% residual activity at 10 mM
EDTA
-
26% inhibition at 1 mM
EDTA
90.8% residual activity at 1 mM
EDTA
-
56.26% residual activity at 1 mM
EDTA
-
71% residual activity at 1 mM
EDTA
68.27% residual activity at 1 mM
EGTA
-
51.4% residual activity at 5 mM
EGTA
-
87.8% residual activity at 10 mM
Fe2+
-
about 70% residual activity at 2 mM
Fe2+
-
72% residual activity at 1 mM
Fe2+
-
the enzyme activity is inhibited by 1 mM Fe2+
Fe2+
-
8.1% residual activity at 50 mM
Fe2+
83.8% residual activity at 1 mM
Fe2+
-
51% residual activity at 1 mM
Fe2+
68.2% residual activity at 5 mM
Fe3+
-
11.4% residual activity at 10 mM
Fe3+
-
9.2% residual activity at 50 mM
Fe3+
-
24.09% residual activity at 1 mM
Fe3+
-
38% residual activity at 1 mM
Fe3+
77.8% residual activity at 5 mM
Hg2+
-
3% residual activity at 10 mM
Hg2+
-
0.3% residual activity at 50 mM
Hg2+
15.35% residual activity at 1 mM
HgCl2
-
1 mM, complete inhibition
HgCl2
-
1 mM, 40.9% inhibition
K+
23.6% residual activity at 100 mM
K+
-
70% residual activity at 100 mM
K+
-
the enzyme activity is inhibited by high concentrations of K+
K+
-
96% residual activity at 10 mM
K+
82.6% residual activity at 1 mM
K+
-
49.13% residual activity at 1 mM
K+
34.6% residual activity at 100 mM
KCl
-
1 mM, 27.4% inhibition
KCl
-
1 mM, 38% inhibition
Li+
-
83.1% residual activity at 10 mM
Li+
92.1% residual activity at 1 mM
Mg2+
-
the enzyme activity is inhibited by 1 mM Mg2+
Mg2+
83.3% residual activity at 1 mM
Mg2+
-
65.93% residual activity at 1 mM
MgCl2
-
1 mM, 15.4% inhibition
MgCl2
-
1 mM, 52.9% inhibition
Mn2+
-
67% residual activity at 1 mM
Mn2+
-
2.5% residual activity at 10 mM
Mn2+
-
53.5% residual activity at 50 mM
Mn2+
-
76.39% residual activity at 1 mM
Mn2+
-
39% residual activity at 1 mM
Mn2+
77.66% residual activity at 1 mM
MnCl2
-
1 mM, 15.4% inhibition
MnCl2
-
1 mM, 36% inhibition
Na+
-
the enzyme activity is inhibited by high concentrations of Na+
Na+
-
90.5% residual activity at 10 mM
Na+
-
the activity is inhibited by more than 50 mM NaCl
Na+
-
85.13%, 84.95% and 46.77% residual activity at 10, 100 and 200 mM, respectively
NaCl
-
above 150 mM
NaCl
-
1.5% or above, complete inhibition
NH4+
-
82% residual activity at 1 mM
NH4+
81.1% residual activity at 1 mM
Ni2+
-
9.7% residual activity at 10 mM
Ni2+
-
49% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
58.6% residual activity at 5 mM
phenylmethylsulfonyl fluoride
-
94.3% residual activity at 10 mM
SDS
22.71% residual activity at 1 mM
SDS
-
61% residual activity at 1 mM
SDS
-
22.6% residual activity at 5 mM
SDS
-
1.8% residual activity at 10 mM
SDS
-
81% inhibition at 1 mM
SDS
21.1% residual activity at 1 mM
SDS
-
56% residual activity at 1 mM
SDS
6.4% residual activity at 1 mM
SDS
16.2% residual activity at 5 mM
Zn2+
-
65% residual activity at 1 mM
Zn2+
-
47.3% residual activity at 10 mM
Zn2+
-
8.3% residual activity at 50 mM
Zn2+
-
almost complete inhibition at 1 mM
Zn2+
35.4% residual activity at 1 mM
Zn2+
-
16.17% residual activity at 1 mM
Zn2+
-
21% residual activity at 1 mM
Zn2+
28% residual activity at 5 mM
additional information
-
not inhibited by Sr2+, Ba2+, K+, Ca2+, Mg2+ or Fe2+
-
additional information
no effect by NEM, iodoacetate, 2-phenanthroline, DTT, 2-mercaptoethanol, N-bromosuccimide, and 4-chloromercuribenzoate
-
additional information
-
no effect by NEM, iodoacetate, 2-phenanthroline, DTT, 2-mercaptoethanol, N-bromosuccimide, and 4-chloromercuribenzoate
-
additional information
EDTA, TritonX-100, Tween-80, methanol, and glycerol have minimal effects on the activity of the enzyme
-
3.2.1.83 kappa-carrageenase
results (
results (
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