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Literature summary for 3.2.1.83 extracted from
- Purification and characterization of a new kappa-carrageenase from a marine Cytophaga-like bacterium (1991), Eur. J. Biochem., 201, 241-247.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | above 150 mM | Bacteria |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.244 | - |
kappa-carrageenan | - |
Bacteria |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | optimal activity at 150 mM | Bacteria | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Bacteria |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteria | - |
strain Dsij, isolated from Delesseria sanguinea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacteria |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacteria |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 16 months | Bacteria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageenan + H2O | - |
Bacteria | additional information | kappa-neocarratetraose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Bacteria |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
2 h, stable | Bacteria |
| 40 | - |
stable for up to 1 h | Bacteria |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
in MOPS buffer | Bacteria |
| 7.2 | - |
in Tris buffer | Bacteria |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5.0: about 50% of maximal activity, pH 8.0: about 75% of maximal activity | Bacteria |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
1 h, about 65% loss of activity | Bacteria |
| 4.5 | - |
1 h, about 40% loss of activity | Bacteria |
| 6 | - |
1 h, about 30% loss of activity | Bacteria |
| 7 | - |
1 h, stable | Bacteria |
| 8.5 | - |
1 h, about 50% loss of activity | Bacteria |
| 9 | - |
1 h, about 75% loss of activity | Bacteria |
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