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Literature summary for 3.2.1.83 extracted from

  • Sun, F.; Ma, Y.; Wang, Y.; Liu, Q.
    Purification and characterization of novel kappa-carrageenase from marine Tamlana sp. HC4 (2010), Chin. J. Oceanol. Limnol., 28, 1139-1145.
No PubMed abstract available
Search for more literature for 3.2.1.83

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 53.25% residual activity at 1 mM Tamlana sp.
Ca2+ 70.84% residual activity at 1 mM Tamlana sp.
Cu2+ 9.67% residual activity at 1 mM Tamlana sp.
EDTA 56.26% residual activity at 1 mM Tamlana sp.
Fe3+ 24.09% residual activity at 1 mM Tamlana sp.
K+ 49.13% residual activity at 1 mM Tamlana sp.
Mg2+ 65.93% residual activity at 1 mM Tamlana sp.
Mn2+ 76.39% residual activity at 1 mM Tamlana sp.
Na+ 85.13%, 84.95% and 46.77% residual activity at 10, 100 and 200 mM, respectively Tamlana sp.
Zn2+ 16.17% residual activity at 1 mM Tamlana sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66400
-
 x * 66400, SDS-PAGE Tamlana sp.

Organism

Organism UniProt Comment Textmining
Tamlana sp.
-
-
-
Tamlana sp. HC4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DE-cellulose 52 anion-exchange chromatography Tamlana sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0774
-
 cell-free culture supernatant, in 25 mM Tris-HCl (pH 8.0) at 30°C Tamlana sp.
6.192
-
 after 80.38fold purification, in 25 mM Tris-HCl (pH 8.0) at 30°C Tamlana sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageenan + H2O the enzyme degrades kappa-carrageenan by hydrolyzing the beta-1,4 linkages to a series of homologous, even numbered oligosaccharides Tamlana sp. kappa-neocarrabiose sulfate + D-galactose
-
 ?
kappa-carrageenan + H2O the enzyme degrades kappa-carrageenan by hydrolyzing the beta-1,4 linkages to a series of homologous, even numbered oligosaccharides Tamlana sp. HC4 kappa-neocarrabiose sulfate + D-galactose
-
 ?

Subunits

Subunits Comment Organism
? x * 66400, SDS-PAGE Tamlana sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Tamlana sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60
-
 Tamlana sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 60 enzyme activity is stable (more than 91%) for 2 h at temperatures of above 45°C. However, 90% of the activity is lost after incubation at 50-60°C for 30 min Tamlana sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Tamlana sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2 8.6 the enzyme is stable over the range of pH 7.2-8.6 below 45°C Tamlana sp.