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Literature summary for 3.2.1.83 extracted from
- Molecular cloning, characterization, and heterologous expression of a new kappa-carrageenase gene from marine bacterium Zobellia sp. ZM-2 (2013), Appl. Microbiol. Biotechnol., 97, 10057-10067.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | - |
Zobellia sp. M-2 |  |
| Triton X-100 | - |
Zobellia sp. M-2 | |
| Tween 80 | - |
Zobellia sp. M-2 | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cgkZ, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His-tagged enzyme with and without signal peptide extracellulary and intracellularly in Escherichia coli strain BL21 (DE3), cloning in Escherichia coli strain DH5alpha | Zobellia sp. M-2 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Zobellia sp. M-2 | |
| Cu2+ | - |
Zobellia sp. M-2 | |
| EDTA | - |
Zobellia sp. M-2 | |
| Mn2+ | - |
Zobellia sp. M-2 | |
| Pb2+ | - |
Zobellia sp. M-2 | |
| SDS | - |
Zobellia sp. M-2 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
kappa-carrageen | pH 6.0, 39°C, recombinant enzyme | Zobellia sp. M-2 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme contains a signal peptide | Zobellia sp. M-2 | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | poor effects by Li+, Mg2+, NH4+, Ca2+ and K+ at 5 mM | Zobellia sp. M-2 | |
| Na+ | activates, best at 100 mM | Zobellia sp. M-2 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37300 | - |
x * 37300, recombinant His-tagged extracellular and intracellular enzyme, SDS-PAGE | Zobellia sp. M-2 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Zobellia sp. M-2 | the enzyme specifically cleaves the internal beta (1-4) linkages of carrageenans and yields a series of homologous even-numbered oligosaccharides | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zobellia sp. M-2 | S5U3X4 | i.e. strain ZM-2, gene cgkZ | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular (24fold) and intracellular (19fold) His-tagged enzyme from Escherichia coli strain BL21 (DE3) cell-free extract and culture supernatant by ammonium sulfate fractionation, dialysis, nickel affinity chromatography and dialysis | Zobellia sp. M-2 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kappa-carrageen + H2O | - |
Zobellia sp. M-2 | ? | - |
? | |
| additional information | the enzyme specifically cleaves the internal beta (1-4) linkages of carrageenans and yields a series of homologous even-numbered oligosaccharides | Zobellia sp. M-2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37300, recombinant His-tagged extracellular and intracellular enzyme, SDS-PAGE | Zobellia sp. M-2 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 39 | - |
- |
Zobellia sp. M-2 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | high activity within this range | Zobellia sp. M-2 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
purified recombinant enzyme, pH 6.0, 3 h, 95% activity remaining | Zobellia sp. M-2 |
| 40 | - |
purified recombinant enzyme, pH 6.0, 1 h, 50% activity remaining | Zobellia sp. M-2 |
| 45 | - |
purified recombinant enzyme, pH 6.0, 15 min, 20% activity remaining | Zobellia sp. M-2 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Zobellia sp. M-2 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | activity range | Zobellia sp. M-2 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
purified recombinant enzyme, 20°C, 2 h, 65% activity remaining | Zobellia sp. M-2 |
| 6 | 8 | purified recombinant enzyme, 20°C, 2 h, 85% activity remaining | Zobellia sp. M-2 |
| 9 | - |
purified recombinant enzyme, 20°C, 2 h, 65% activity remaining | Zobellia sp. M-2 |
| 10 | - |
purified recombinant enzyme, 20°C, 2 h, inactivation | Zobellia sp. M-2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 16, GH16 | Zobellia sp. M-2 |
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