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Literature summary for 3.2.1.83 extracted from

  • Ma, Y.; Dong, S.; Jiang, X.; Li, J.; Mou, H.
    PURIFICATION AND CHARACTERIZATION OF ?-CARRAGEENASE FROM MARINE BACTERIUM MUTANT STRAIN PSEUDOALTEROMONAS SP. AJ5-13 AND ITS DEGRADED PRODUCTS (2010), J. Food Biochem., 34, 661-678.
    View publication on PubMed
Search for more literature for 3.2.1.83

Inhibitors

Inhibitors Comment Organism Structure
Co2+ almost complete inhibition at 1 mM Pseudoalteromonas sp.
Cu2+ almost complete inhibition at 1 mM Pseudoalteromonas sp.
EDTA 26% inhibition at 1 mM Pseudoalteromonas sp.
additional information not inhibited by Sr2+, Ba2+, K+, Ca2+, Mg2+ or Fe2+ Pseudoalteromonas sp.
Na+ the activity is inhibited by more than 50 mM NaCl Pseudoalteromonas sp.
SDS 81% inhibition at 1 mM Pseudoalteromonas sp.
Zn2+ almost complete inhibition at 1 mM Pseudoalteromonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ the activity is optimum in the presence of 50 mM NaCl Pseudoalteromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Pseudoalteromonas sp.

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp.
-
-
-
Pseudoalteromonas sp. AJ5-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, CM-cellulose 52 column chromatography, and Sephadex G-200 gel filtration Pseudoalteromonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61
-
 cell-free culture supernatant, in 25 mM Tris-HCl (pH 8.0) at 32°C Pseudoalteromonas sp.
5778
-
 after 95fold purification, in 25 mM Tris-HCl (pH 8.0) at 32°C Pseudoalteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageenan + H2O
-
 Pseudoalteromonas sp. kappa-neocarrabiose sulfate + kappa-neocarratetraose sulfate + kappa-neocarrahexaose sulfate + kappa-neocarraoctaose sulfate + kappa-neocarradecaose sulfate + D-galactose main end-products ?
kappa-carrageenan + H2O
-
 Pseudoalteromonas sp. AJ5-1 kappa-neocarrabiose sulfate + kappa-neocarratetraose sulfate + kappa-neocarrahexaose sulfate + kappa-neocarraoctaose sulfate + kappa-neocarradecaose sulfate + D-galactose main end-products ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Pseudoalteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Pseudoalteromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
28 75 the enzyme is stable at 28°C, but 95% of the activity is lost at 50-75°C for 30 min Pseudoalteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudoalteromonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.6 8.6
-
 Pseudoalteromonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudoalteromonas sp. isoelectric focusing
-
 8.5