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Literature summary for 3.2.1.83 extracted from

  • Khambhaty, Y.; Mody, K.; Jha, B.
    Purification and characterization of k-carrageenase from a novel gamma-proteobacterium, Pseudomonas elongata (MTCC 5261) syn. Microbulbifer elongatus comb. Nov (2007), Biotechnol. Bioprocess Eng., 12, 668-675.
No PubMed abstract available
Search for more literature for 3.2.1.83

General Stability

General Stability Organism
repeated freezing and thawing drastically reduces activity Microbulbifer elongatus

Inhibitors

Inhibitors Comment Organism Structure
AlCl3 1 mM, 39.7% inhibition Microbulbifer elongatus
BaCl2 1 mM, 26.8% inhibition Microbulbifer elongatus
CaCl2 1 mM, 50.7% inhibition Microbulbifer elongatus
FeCl3 1 mM, 36.5% inhibition Microbulbifer elongatus
HgCl2 1 mM, 40.9% inhibition Microbulbifer elongatus
KCl 1 mM, 38% inhibition Microbulbifer elongatus
MgCl2 1 mM, 52.9% inhibition Microbulbifer elongatus
MnCl2 1 mM, 36% inhibition Microbulbifer elongatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for kappa-carrageenan is 6.66 mg/ml Microbulbifer elongatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Microbulbifer elongatus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
128000
-
 gel filtrration Microbulbifer elongatus

Organism

Organism UniProt Comment Textmining
Microbulbifer elongatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Microbulbifer elongatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
426.2
-
-
 Microbulbifer elongatus

Storage Stability

Storage Stability Organism
20°C, 5 days, 40% loss of activity Microbulbifer elongatus
frozen, stable for 15 days Microbulbifer elongatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kappa-carrageenan + H2O
-
 Microbulbifer elongatus ?
-
 ?
additional information isolated kappa-carrageenase can successfully generate protoplasts of Kappaphycus alvarezii. No activity with lambda-carrageenan, ioro-carrageenan and LMP agarose Microbulbifer elongatus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 128000, SDS-PAGE Microbulbifer elongatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Microbulbifer elongatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 40% loss of activity within 5 days, complete loss of activity within 12 days Microbulbifer elongatus
37
-
 5 days, 50% loss of activity Microbulbifer elongatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
 and a second optimum at pH 7.7 Microbulbifer elongatus
7.7
-
 and a second optimum at pH 5.6 Microbulbifer elongatus

pH Range

pH Minimum pH Maximum Comment Organism
4.8 9 pH 4.8: about 70% of maximal activity, pH 9.0: about 60% of maximal activity Microbulbifer elongatus