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3.2.1.3: glucan 1,4-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.3

Reaction

(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose
+
H2O
=
(alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose
 +
beta-D-glucopyranose

Synonyms

1,4-alpha-D-glucan glucohydrolase, 1,4-alpha-D-glucan-glucohydrolase, acid maltase, alpha-(1,4)-D-glucan glucohydrolase, alpha-1,4-D-glucan glucohydrolase, alpha-1,4-glucan glucohydrolase, AMG, AmyA, AmyC, AmyD, amyloglucosidase, AnGA, APGA1, AtriGA15A, exo-1,4-alpha-D-glucan glucanohydrolase, exo-1,4-alpha-D-glucanohydrolase, exo-1,4-alpha-glucosidase, exo-amylase, GA, GA A, GA1, GA2, GAI, GAII, GAM, GAM-1, GAM-2, gamma-amylase, GAMP, GHF15 glucoamylase, GLA, Gla1, GlaA, GLL1, Glu-1.1, Glu-A, Glu1, GlucaM, Glucan 1,4-alpha-glucosidase, glucoamylase, glucoamylase 1, glucoamylase 2, glucoamylase C, glucoamylase D, glucoamylase G1, glucoamylase GA15A, glucoamylase P, glucose amylase, GluR, glycoamylase, HjGA, HrGA, lysosomal alpha-glucosidase, maltase glucoamylase, maltase-glucoamylase, maltooligosaccharide-metabolizing enzyme, Meiotic expression upregulated protein 17, MGA, MGAM, More, PDE_05527, PoGA, PoGA15A, PoxGA15A, raw starch-degrading enzyme, raw starch-degrading glucoamylase, RSDE, RSDG, SBD, Sga1, SSG, Sta1, Sta1p, starch-digesting glucoamylase, TagA, tGA, TmGLA, TmGlu1, TtcGA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.3 glucan 1,4-alpha-glucosidase

General Stability

General Stability on EC 3.2.1.3 - glucan 1,4-alpha-glucosidase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
5 M guanidine hydrochloride, almost complete denaturation
-
8 M urea, partial denaturation
-
after treatment with 4 M guanidinium chloride, gel filtration on Sephadex G-25 column results in 50% loss of activity
-
after treatment with 8 M urea, gel filtration on Sephadex G-25 column results in complete recovery of activity
-
Ca2+ and Na+ highly increase the stability of the enzyme
-
CaCl2 at 1 mM stabilizes the purified native enzyme
-
denaturation and renaturation does not seem to offer an economical approach to improve the usage time of immobilized enzyme
-
high operational stability of the enzyme immobilized onto poly(2-hydroxyethyl methacrylate)/ethylene glycol dimetharylate microspheres
-
modification of the carbohydrate component by adding 1-deoxymannonojirimycin to the culture medium induces inhibition of alpha-mannosidase involved in the processing, leading to a more complete glycosylation and consequently to a higher stability of the enzyme
-
stable to treatment with 1.5% SDS
-
starch at 10% stabilizes the purified enzyme at 55°C
-
the EDTA-coupled enzyme is more stable towards proteases and urea
-
the purified enzyme is sensitive to proteolytic degradation by alpha-chymotrypsin, half-life 39 min
-
the recombinant enzyme stability is enhanced by 27% by Ca2+ at 0.1 mM
-