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3.2.1.3: glucan 1,4-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.3

Reaction

(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose
+
H2O
=
(alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose
+
beta-D-glucopyranose

Synonyms

1,4-alpha-D-glucan glucohydrolase, 1,4-alpha-D-glucan-glucohydrolase, acid maltase, alpha-(1,4)-D-glucan glucohydrolase, alpha-1,4-D-glucan glucohydrolase, alpha-1,4-glucan glucohydrolase, AMG, AmyA, AmyC, AmyD, amyloglucosidase, AnGA, APGA1, AtriGA15A, exo-1,4-alpha-D-glucan glucanohydrolase, exo-1,4-alpha-D-glucanohydrolase, exo-1,4-alpha-glucosidase, exo-amylase, GA, GA A, GA1, GA2, GAI, GAII, GAM, GAM-1, GAM-2, gamma-amylase, GAMP, GHF15 glucoamylase, GLA, Gla1, GlaA, GLL1, Glu-1.1, Glu-A, Glu1, GlucaM, Glucan 1,4-alpha-glucosidase, glucoamylase, glucoamylase 1, glucoamylase 2, glucoamylase C, glucoamylase D, glucoamylase G1, glucoamylase GA15A, glucoamylase P, glucose amylase, GluR, glycoamylase, HjGA, HrGA, lysosomal alpha-glucosidase, maltase glucoamylase, maltase-glucoamylase, maltooligosaccharide-metabolizing enzyme, Meiotic expression upregulated protein 17, MGA, MGAM, More, PDE_05527, PoGA, PoGA15A, PoxGA15A, raw starch-degrading enzyme, raw starch-degrading glucoamylase, RSDE, RSDG, SBD, Sga1, SSG, Sta1, Sta1p, starch-digesting glucoamylase, TagA, tGA, TmGLA, TmGlu1, TtcGA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.3 glucan 1,4-alpha-glucosidase

Specific Activity

Specific Activity on EC 3.2.1.3 - glucan 1,4-alpha-glucosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.3
substrate maltose, pH 4.7, 40°C
1.02
-
pancreas
1.8
-
purified recombinant enzyme
10.44
-
cell culture
102.2
-
suspension culture
11.2
purified native enzyme, pH 4.5, 40°C, substrate raw cassava starch
11.7
-
crosslinked glucoamylase crystals
1125
-
mutant enzyme
129.82
-
purified enzyme, pH 5.5, 50°C
13.2
purified enzyme, pH 5.0, 60°C, with soluble starch as substrate
13.26
-
crude enzyme extract
13.3
-
recombinant mutant S30P/T62A/H391Y
13.6
-
recombinant mutant T62A
14
-
recombinant mutant T290A
14.2
-
recombinant wild-type enzyme
16.25
-
purified enzyme
16.73
Thermochaetoides thermophila
recombinant enzyme in Pichia pastoris
16.8
-
recombinant mutant S119P
16.9
-
recombinant mutant D20C/A27C/S30P/G137A
165.8
-
purified native enzyme, pH 5.6, 50°C
17.7
-
recombinant mutant H391Y
17.9
-
recombinant mutant S30P/T290A
18.1
-
recombinant mutant S30P/T290A/H391Y
18.73
substrate pullalan, pH 5.0, 45°C
180
purified recombinant enzyme, pH 7.0, 50°C
19.5
-
recombinant mutant S30P/H391Y
19.73
-
purified enzyme, substrate starch
2.54
-
culture supernatant, substrate starch
200
-
purified commercial preparation
216
-
purified wild-type enzyme, pH 5.0, 40°C, substrate starch
22.7
purified native enzyme, pH 4.5, 40°C, substrate raw corn starch
2300.5
-
purified extracellular enzyme, pH 7.0, 40°C
24.7
purified native enzyme, pH 4.5, 40°C, substrate raw rice starch
25
substrate starch, pH 4.7, 30°C
25.9
-
purified isozyme 1, pH 5.5, 37°C
26 - 39
-
immobilized glucoamylase
29
-
enzyme form G4
297.3
-
purified native enzyme
298
purified recombinant enzyme, pH 4.5, 60°C, substarte raw potato starch
3 - 8
30.14
substrate soluble potato starch, pH 5.0, 45°C
300
-
purified commercial preparation
301.77
-
purified native extracellular enzyme, pH 5.0, 65°C
32.6
-
glucoamylase M1
34
-
partially purified enzyme
34.3
substrate amylose, pH 5.0, 45°C
36.73
substrate amylopectin, pH 5.0, 45°C
37.1
beta domain plus catalytic domain independently expressed, pH 4.6, 70°C
377.4
-
glucoamylase I
39
-
enzyme form G2
39.64
substrate soluble wheat starch, pH 5.0, 45°C
4.21
-
purified recombinant enzyme, substrate amylopectin
4.5
purified recombinant enzyme mutant lacking the starch-binding domain, pH 4.5, 40°C, substrate raw rice starch
4.8
substrate amylopectin, pH 4.7, 58°C
40.8
-
glucoamylase M2
406.9
-
purified native enzyme
41
-
enzyme form G1
43.8
-
substrate starch, pH 5.5, temperature not specified in the publication
44
-
enzyme form G4
457.5
-
glucoamylase II
46.8
-
purified enzyme
465
-
purified enzyme, substrate starch
47
-
enzyme form G5
495.2
-
purified mutant enzyme, pH 5.0, 40°C, substrate starch
5.1
purified recombinant enzyme mutant lacking the starch-binding domain, pH 4.5, 40°C, substrate raw potato starch
5.2
substrate maltose, pH 4.7, 40°C
5.3
purified recombinant enzyme mutant lacking the starch-binding domain, pH 4.5, 40°C, substrate raw cassava starch
5.6
-
soluble glucamylase
5.76 - 8.86
-
liposome-entrapped enzyme
56.3
purified recombinant enzyme
560
-
parent strain
57.9
purified recombinant enzyme mutant lacking the starch-binding domain, pH 4.5, 40°C, substrate soluble starch
6.8
purified recombinant enzyme mutant lacking the starch-binding domain, pH 4.5, 40°C, substrate raw corn starch
600
-
after purification
61.34
-
glycated purified enzyme
65.64
-
native purified enzyme
6619
-
purified enzyme, pH 6.0, 60°C
690
Arachniotus sp.
-
-
7.8
substrate amylose, pH 4.7, 58°C
71.2
Thermochaetoides thermophila
-
purified enzyme
74.5
-
purified isozyme 2, pH 5.5, 37°C
758
endophytic fungus EF6
-
purified enzyme, substrate is soluble starch, pH 6.0, 50°C
80.5
purified native enzyme, pH 4.5, 40°C, substrate soluble starch
839.1
-
purified enzyme
89
-
pH 5.5, 65°C
9.4
-
substrate starch, pH 5.5, temperature not specified in the publication
9.5
substrate amylopectin, pH 4.7, 58°C
9.9
purified native enzyme, pH 4.5, 40°C, substrate raw potato starch
94.7
-
callus
95
-
pH 5.0, 40°C
additional information