Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.3: glucan 1,4-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.3

Reaction

(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose
+
H2O
=
(alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose
 +
beta-D-glucopyranose

Synonyms

1,4-alpha-D-glucan glucohydrolase, 1,4-alpha-D-glucan-glucohydrolase, acid maltase, alpha-(1,4)-D-glucan glucohydrolase, alpha-1,4-D-glucan glucohydrolase, alpha-1,4-glucan glucohydrolase, AMG, AmyA, AmyC, AmyD, amyloglucosidase, AnGA, APGA1, AtriGA15A, exo-1,4-alpha-D-glucan glucanohydrolase, exo-1,4-alpha-D-glucanohydrolase, exo-1,4-alpha-glucosidase, exo-amylase, GA, GA A, GA1, GA2, GAI, GAII, GAM, GAM-1, GAM-2, gamma-amylase, GAMP, GHF15 glucoamylase, GLA, Gla1, GlaA, GLL1, Glu-1.1, Glu-A, Glu1, GlucaM, Glucan 1,4-alpha-glucosidase, glucoamylase, glucoamylase 1, glucoamylase 2, glucoamylase C, glucoamylase D, glucoamylase G1, glucoamylase GA15A, glucoamylase P, glucose amylase, GluR, glycoamylase, HjGA, HrGA, lysosomal alpha-glucosidase, maltase glucoamylase, maltase-glucoamylase, maltooligosaccharide-metabolizing enzyme, Meiotic expression upregulated protein 17, MGA, MGAM, More, PDE_05527, PoGA, PoGA15A, PoxGA15A, raw starch-degrading enzyme, raw starch-degrading glucoamylase, RSDE, RSDG, SBD, Sga1, SSG, Sta1, Sta1p, starch-digesting glucoamylase, TagA, tGA, TmGLA, TmGlu1, TtcGA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.3 glucan 1,4-alpha-glucosidase

Cloned

Cloned on EC 3.2.1.3 - glucan 1,4-alpha-glucosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of gene glaA encoding glucoamylase G1 of Aspergillus tubingensis strains 643.92 and 127.49 reveals high identity with the gene/enzyme from Aspergillus niger strain DSM 823, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
construction of combinatorial library of starch-binding domain of the enzyme, expression in Escherichia coli strain DH5alpha, screening of clones with enhanced activity by yeast display method using Saccharomyces cerevisiae strain MT8-1, overview
-
DNA and amino acid sequence determination and analysis, constitutive overexpression of the His-tagged enzyme in Escherichia coli
-
DNA and amino acid sequence determination and analysis, sequence comparisons
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
DNA and amino acid sequence determination and anaylsis of wild-type and mutant enzymes, expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain C468 using vector YEpPM18
-
DNA and amino acid sequence determination, analysis and comparisons
-
expressed in Saccharomyces cerevisiae
expressed in transgenic rice seeds
expression in Aspergillus niger
expression in Aspergillus oryzae
expression in Escherichia coli strain MV1184
-
expression in Escherichia coli, as fusion protein with Staphylococcus protein A
expression in Penicillium canescens
expression in Pichia pastoris
expression in Pichia pastoris, Saccharomyces cerevisiae and Aspergillus niger
-
expression in Pichia pastoris, Saccharomyces cerevisiae or Aspergillus niger. In Pichia pastoris the C320A/E400C double mutant is produced at 75% of the wild-type glucoamylase, while the corresponding single mutants are produced at 1% and 20% of the wild-type level, respectively
-
expression in Saccharomyces cerevisae
expression in Saccharomyces cerevisiae
expression in Saccharomyces cerevisiae strain MT8-1 on the cell surface, subcloning in Escherichia coli
-
expression in Saccharomyces cerevisiae using the yeast phosphoglycerate kinase gene promoter with the original vector signal sequence eliminated, co-expression with a barley alpha-amylase 1 mutant with constitutive secretion in active forms
-
expression in Saccharomyces cerevisiae, secretion of functional enzyme to the medium from recombinant cells
-
expression in yeast
-
expression of active MGA N-terminal catalytic domain MGAnt, residues 87-955, in COS-1 cells, and overexpression of MGAnt in Drosophila S2 cells, secretion of recombinant proteins
-
expression of glucoamylase starch binding domain in Escherichia coli
expression of His-tagged N-terminal subunit of human small intestinal enzyme in S2 cells, secretion to the cell culture medium
-
expression of the chimeric mutant enzyme in Saccharomyces cerevisiae strain Y428
-
expression of the enzyme's isolated carbohydrate-binding module CBM21 in Escherichia coli strain BL21
expression of wild-type and mutant enzymes in Escherichia coli strain MV1184
-
expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain AH22, secretion of recombinant enzymes
expression of wild-type and mutant S54P/T314A/H415Y enzymes in Saccharomyces cerevisiae
-
expression of wild-type enzyme in Saccharomyces cerevisiae strain C468, cloning and expression of mutant enzymes in Escherichia coli strain DH5alpha
-
gene APGA1, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris
gene GAA, DNA and amino acid sequence determination and analysis, phylogenic tree, the N-terminal 17 amino acids are presumed to be a signal peptide, recombinant methanol-induced expression of FLAG-tagged enzyme in Pichia pastoris strain GS115
gene GAMP, recombinant expression of the enzyme in Aspergillus niger strain MBin118
gene gla, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional overexpression in Pichia pastoris strain GS115, secretion to the culture supernatant
Thermochaetoides thermophila
gene gla1, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris, phylogenetic analysis
gene gla66, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae JDS+S, analysis of expression and translational control
gene glaA, DNA and amino acid sequence determination and analysis, the gene encoding glucoamylase G1 of Aspergillus niger strain DSM 823 shows high identity with the gene from Aspergillus tubingensis strains 643.92 and 127.49, phylogenetic analysis
gene GLAA, recombinant expression of the enzyme in Aspergillus niger strain MBin118
gene PDE_05527, recombinant expression of the enzyme in Aspergillus niger strain MBin118
gene PoGA15A, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of codon-optimized His-tagged enzyme the sequence coding for the signal peptide in Pichia pastoris strain GS115 under control of AOX1 promoter. The recombinant enzyme is secreted
gene ssg, phylogenetic analysis, expression in Escherichia coli
gene STA1, DNA and amino acid sequence determination and analysis, homology modelling
gene taGA, DNA and amino acid sequence determination and anaylsis, expression in Escherichia coli
-
gene TmGlu1, DNA and amino acid sequeence determination and analysis, recombinant secretory expression of the enzyme in Pichia pastoris strain X-33
genes amyC and amyD, expression in Pichia pastoris strain X33
-
introduction of extra copies of gene encoding for Humicola glucoamlyase into the genome of Humicola strains
-
overexpression in Aspergillus niger
-
overexpression of the N-terminal catalytic subunit in Drosophila melanogaster S2 cells and secretion of the recombinant protein from the cells
phylogenetic tree, expression of the intracellular enzyme in Escherichia coli
recombinant expression of the glucoamylase subunit Ct-MGAM splice form N20 and the sucrase subunit Ct-SI in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
recombinant expression of the starch-binding domain (SBD)-containing polypeptide (amino acids 509-616) of glucoamylase in Escherichia coli
recombinant secretory overexpression of the raw starch-degrading glucoamylase PoxGA15A in Penicillium oxalicum strain strain HP7-1 and DELTAPoxPepA HP7-1 derivative protoplasts using strong promoter and signal peptide, transcriptome-based selection of strongly inducible promoters in Penicillium oxalicum, and secretome-based selection of signal peptides related to protein secretion in Penicillium oxalicum, overview