Application | Comment | Organism |
---|---|---|
industry | the enzyme can be useful in industrial processing and hydrolysis of starch | Gymnascella citrina |
General Stability | Organism |
---|---|
the purified enzyme is sensitive to proteolytic degradation by alpha-chymotrypsin, half-life 39 min | Gymnascella citrina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the purified enzyme is sensitive to proteolytic degradation by alpha-chymotrypsin | Gymnascella citrina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
starch | pH 3.5-5.5, 50°C | Gymnascella citrina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Gymnascella citrina | - |
starch + beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gymnascella citrina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 63fold to homogeneity by ammonium sulfate precipitation, anion exchange and hydrophobic interaction chromatography, and again anion exchange chromatography | Gymnascella citrina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | solid-state culture on wheat bran | Gymnascella citrina | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Gymnascella citrina | starch + beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exo-1,4-alpha-D-glucan glucanohydrolase | - |
Gymnascella citrina |
glucoamylase | - |
Gymnascella citrina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
at 0.39 mg/ml purified enzyme | Gymnascella citrina |
55 | - |
at 1 mg/ml purified enzyme | Gymnascella citrina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life of purified enzyme is 7 min, irreversible inactivation | Gymnascella citrina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
starch | pH 3.5-5.5, 50°C | Gymnascella citrina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.5 | - |
Gymnascella citrina |