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3.2.1.3: glucan 1,4-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.3

Reaction

(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose
+
H2O
=
(alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose
+
beta-D-glucopyranose

Synonyms

1,4-alpha-D-glucan glucohydrolase, 1,4-alpha-D-glucan-glucohydrolase, acid maltase, alpha-(1,4)-D-glucan glucohydrolase, alpha-1,4-D-glucan glucohydrolase, alpha-1,4-glucan glucohydrolase, AMG, AmyA, AmyC, AmyD, amyloglucosidase, AnGA, APGA1, AtriGA15A, exo-1,4-alpha-D-glucan glucanohydrolase, exo-1,4-alpha-D-glucanohydrolase, exo-1,4-alpha-glucosidase, exo-amylase, GA, GA A, GA1, GA2, GAI, GAII, GAM, GAM-1, GAM-2, gamma-amylase, GAMP, GHF15 glucoamylase, GLA, Gla1, GlaA, GLL1, Glu-1.1, Glu-A, Glu1, GlucaM, Glucan 1,4-alpha-glucosidase, glucoamylase, glucoamylase 1, glucoamylase 2, glucoamylase C, glucoamylase D, glucoamylase G1, glucoamylase GA15A, glucoamylase P, glucose amylase, GluR, glycoamylase, HjGA, HrGA, lysosomal alpha-glucosidase, maltase glucoamylase, maltase-glucoamylase, maltooligosaccharide-metabolizing enzyme, Meiotic expression upregulated protein 17, MGA, MGAM, More, PDE_05527, PoGA, PoGA15A, PoxGA15A, raw starch-degrading enzyme, raw starch-degrading glucoamylase, RSDE, RSDG, SBD, Sga1, SSG, Sta1, Sta1p, starch-digesting glucoamylase, TagA, tGA, TmGLA, TmGlu1, TtcGA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.3 glucan 1,4-alpha-glucosidase

Molecular Weight

Molecular Weight on EC 3.2.1.3 - glucan 1,4-alpha-glucosidase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
105000
-
gel filtration
105360
-
x * 105360, recombinant N-terminal catalytic domain, MALDI-TOF mass spectrometry
116000
-
1 * 116000 + 1 * 212000, SDS-PAGE
128000
-
1 * 128000, glucoamylase II, SDS-PAGE
130000
-
about, recombinant enzyme, native PAGE
133000
-
x * 133000 + x * 90000, SDS-PAGE
140000
141000
-
x * 141000 + x * 95000, SDS-PAGE
150000
-
2 * 150000, glucoamylase I, SDS-PAGE
155000
-
gel filtration
16000 - 46000
-
gel filtration
186000
-
2 * 186000, SDS-PAGE
212000
-
1 * 116000 + 1 * 212000, SDS-PAGE
225000
-
gel filtration
230000
-
glucoamylase I, gel filtration
250000
-
SDS-PAGE, assumed
26850
292000
recombinant intracellular enzyme, native PAGE
33000
-
1 * 65000 + 1 * 33000, SDS-PAGE
40000
41000
-
gel filtration
42000
440000
-
papain solubilized enzyme, gel filtration
47000
-
SDS-PAGE, assumed
48000
48500
-
SDS-PAGE, assumed
49000
51000
-
enzyme form GA-II, gel filtration
52000
-
enzyme form GA-I, gel filtration
53000
-
1 * 53000, enzyme form GA-I, SDS-PAGE
54000
55000
550000
-
detergent solubilized enzyme, gel filtration
56000
56583
-
x * 56000, SDS-PAGE, x * 56583, mass spectrometry
57000
57151
-
x * 62000, SDS-PAGE, x * 57151, mass spectrometry
58000
58600
-
glucoamylase 2, ultracentrifugal equilibrium sedimentation
59000
60000
61000
61400
-
glucoamylase 3, ultracentrifugal equilibrium sedimentation
62000
62031
endophytic fungus EF6
-
1 * 62200, SDS-PAGE, 1 * 62031, mass spectrometry
62200
endophytic fungus EF6
-
1 * 62200, SDS-PAGE, 1 * 62031, mass spectrometry
62800
x * 62800, deduced from nucleotide sequence
63000
63900
gel filtration
64000
65000
65400
Thermochaetoides thermophila
-
gel filtration and native PAGE
66000
67000
-
glucoamylase II, gel filtration
67500
-
1 * 67500, SDS-PAGE
68000
-
experimental molecular weight for glucoamylase 2, excellent agreement with the theoretical value
68400
69000
70000
71600
-
gel filtration
72000
72000 - 74000
-
gel filtration
72063
-
1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry
72800
-
gel filtration
72876
-
1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry
73000
74000
75000
75400
about, native PAGE
76000
77000
77600
-
x * 77600, SDS-PAGE
78000
79000
-
1 * 79000, glucoamylase G3, SDS-PAGE
79500
-
equilibrium sedimentation
79920
-
x * 77000, recombinant enzyme, SDS-PAGE, x * 79920, amino acid sequence calculation
80370
-
MALDI-MS
81000
-
gel filtration
82000
82327
-
x * 82327, mass spectroscopy, recombinant GA expressed in Pichia pastoris
82330
-
recombinant enzyme expressed in Pichia pastoris, matrix-assisted laser desorption ionization mass spectrometry
82839
-
x * 82839, mass spectroscopy, recombinant GA expressed in Aspergillus niger
82840
-
recombinant enzyme expressed in Aspergillus niger, MALDI-MS
83000
83700
-
calculation from diffusion and sedimentation data
83869
-
x * 83869, mass spectroscopy, recombinant GA expressed in Saccharomyces cerevisiae
83870
-
recombinant enzyme expressed in Saccharomyces cerevisiae, matrix-assisted laser desorption ionization mass spectrometry
84000
-
glucoamylase I, calculation from diffusion and sedimentation data
85000
86000
-
glucoamylase II, calculation from diffusion and sedimentation data
86500
-
x * 86500, SDS-PAGE
87000
88000
89000
-
x * 60000, isozyme GA-I, SDS-PAGE, x * 89000, isozyme GA-II, SDS-PAGE
90000
91000
92000
x * 92000, extracellular enzyme
93000
-
1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry
94000
-
experimental molecular weight for GA1:L0
95000
-
x * 141000 + x * 95000, SDS-PAGE
96000
-
x * 96000, glucoamylase II, SDS-PAGE
98000
-
native extracellular enzyme, native PAGE
additional information