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3.2.1.3: glucan 1,4-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,4-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.3

Reaction

(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose
+
H2O
=
(alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose
 +
beta-D-glucopyranose

Synonyms

1,4-alpha-D-glucan glucohydrolase, 1,4-alpha-D-glucan-glucohydrolase, acid maltase, alpha-(1,4)-D-glucan glucohydrolase, alpha-1,4-D-glucan glucohydrolase, alpha-1,4-glucan glucohydrolase, AMG, AmyA, AmyC, AmyD, amyloglucosidase, AnGA, APGA1, AtriGA15A, exo-1,4-alpha-D-glucan glucanohydrolase, exo-1,4-alpha-D-glucanohydrolase, exo-1,4-alpha-glucosidase, exo-amylase, GA, GA A, GA1, GA2, GAI, GAII, GAM, GAM-1, GAM-2, gamma-amylase, GAMP, GHF15 glucoamylase, GLA, Gla1, GlaA, GLL1, Glu-1.1, Glu-A, Glu1, GlucaM, Glucan 1,4-alpha-glucosidase, glucoamylase, glucoamylase 1, glucoamylase 2, glucoamylase C, glucoamylase D, glucoamylase G1, glucoamylase GA15A, glucoamylase P, glucose amylase, GluR, glycoamylase, HjGA, HrGA, lysosomal alpha-glucosidase, maltase glucoamylase, maltase-glucoamylase, maltooligosaccharide-metabolizing enzyme, Meiotic expression upregulated protein 17, MGA, MGAM, More, PDE_05527, PoGA, PoGA15A, PoxGA15A, raw starch-degrading enzyme, raw starch-degrading glucoamylase, RSDE, RSDG, SBD, Sga1, SSG, Sta1, Sta1p, starch-digesting glucoamylase, TagA, tGA, TmGLA, TmGlu1, TtcGA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.3 glucan 1,4-alpha-glucosidase

Purification

Purification on EC 3.2.1.3 - glucan 1,4-alpha-glucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2.53fold by DEAE-Fractogel and Concanacalin A-Sepharose chromatography
-
3 enzyme forms: GI, GII and GIII
-
5 forms of glucoamylase: G1-G5
-
affinity chromatography on acarbose-Sepharose, Q-Sepharose
-
affinity chromatography, ultrafiltration and ion exchange
-
ammonium sulfate fractionation, gel filtration
-
ammonium sulfate precipitation and gel filtration
-
ammonium sulfate precipitation, anion exchange, gel filtration, hydrophobic interaction chromatography
-
ammonium sulfate precipitation, anion exchange, hydrophobic interaction chromatography
Thermochaetoides thermophila
-
ammonium sulfate precipitation, gel filtration and ion exchange
ammonium sulfate precipitation, gel filtration, affinity chromatography
-
ammonium sulfate precipitation, ion exchange
-
ammonium sulfate, G-25 Sephadex, Mono Q, Econo-Pac S, TSK 2000 Gel
-
ammonium sulfate, Q-Sepharose, Mono Q, gel filtration
Arachniotus sp.
-
ammonium sulfate, Sephadex G-25
-
anion exchange and gel filtration chromatographie
-
anion exchange, gel filtration
-
crude glucoamylase preparation is partially purified by acetone precipitation (80% saturation) and is used in enzyme assays
-
DEAE-cellulose, ultrafiltration, CM-cellulose
-
ethanol precipitation
-
ethanol precipitation and affinity chromatography using acarbose
-
ethanol precipitation, ion exchange and gel filtration
-
extracellular enzyme 1.32fold from crude cell extract by anion exchange chromatography and dialysis
-
further purification of the commercial preparation by anion exchange chromatography
-
gel filtration
glucoamylase I and II
glucoamylase M1
-
glucoamylase M2
-
glucoamylase starch binding domain, affinity purification on granular corn starch
glucoamylases from a wild-type and a deoxy-D-glucose-resistant mutant Aspergillus niger to apparent homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography, the wild-tyype enzyme is purified 25.4fold, the mutant 30.6fold
-
ion exchange and gel filtration
ion exchange, gel filtration
lyophilization, acetone precipitation, SP-Sepharose, Sephadex G-50
-
membrane filtration and gel filtration
Cephalosporium eichhorniae
-
native enzyme 14.49fold from culture medium by ammonium sulfate fractionation, anion exchange chromatography and gel filtration
endophytic fungus EF6
-
native enzyme 18fold from viscus, by ammonium sulfate fractionation, alternating gel filtration and ion exchange chromatography steps, to homogeneity
-
native enzyme 24fold by ammonium sulfate fractionation, dialysis, cation exchange chromatography, ultrafiltration, and gel filtration, to homogeneity
-
native enzyme 47.9fold by anion exchange chromatography and gel filtration
-
native enzyme 5.0fold by ammonium sulfate fractionation, gel filtration, and cation and anion exchange chromatography to homogeneity
-
native enzyme 60.61fold by ethanol precipitation, hydrophobic interaction chromatography, and cation exchange chromatography, recombinant secreted His-tagged truncated enzyme from Pichia pastoris strain GS115 culture supernatant by nickel affinity chromatography
native enzyme 63.3fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography to homogeneity
-
native enzyme 63fold to homogeneity by ammonium sulfate precipitation, anion exchange and hydrophobic interaction chromatography, and again anion exchange chromatography
-
native enzyme 68.2% by dialysis, gel filtration and anion exchange chromatography, more than 90% of native GA A binds to raw starch
-
native enzyme 93fold from pancreas to homogeneity by glycogen precipitation, ion exchange chromatography, and gel filtration
-
native enzyme by ion exchange and hydrophobic interaction chromatography
-
native enzyme partially 10.2fold by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography
-
native enzyme to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration
-
native extracellular enzyme 2.73fold from submerged culture by starch adsorption chromatography and gel filtration
-
native extracellular enzyme 26.2fold to homogeneity by ammonium sulfate precipitation, anion exchange chromatography, and hydrophobic interaction chromatography, followed by gel filtration
-
native extracellular enzyme 33.2fold by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, and gel filtration
native extracellular enzyme 39fold to homogeneity by ammonium sulfate precipitation, anion exchange chromatography, and hydrophobic interaction chromatography
-
native extracellular enzyme 53.64fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, ion exchange chromatography, and native PAGE
native extracellular enzyme from culture supernatant 7.3fold to homogeneity by ammonium sulfate fractionation and gel filtration
-
native isozymes 120fold by starch affinity chromatography, isozyme separation by PAGE zymography
-
native isozymes GA-I and GA-II
-
native soluble enzyme from culture medium 16.3 fold to homogeneity by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration
Thermochaetoides thermophila
-
partially purification to 30fold homogeneity by heat treatment and gel filtration chromatography
-
precipitation with PEG, affinity chromatography, chromatofocusing
-
recombinant AmyC and AmyD from Pichia pastoris strain X33 culture supernatant by affinity chromatography, the recombinant enzymes are deglycosylated with endoglycosidase H and PNGase F
-
recombinant enzyme 22fold from Escherichia coli to homogeneity using heat treatment, anion exchange chromatography, and gel filtration
-
recombinant enzyme 80.9fold from Escherichia coli by heat treatment at 70°C, hydrophobic interaction chromatography, and gel filtration
recombinant enzyme from Aspergillus niger strain MBin118 by alpha-cyclodextrin affinity chromatography, elution with beta-cyclodextrin, followed by dialysis and ultrafiltration, to homogeneity
recombinant enzyme from Aspergillus niger strain MBin118 by alpha-cyclodextrin affinity chromatography, elution with beta-cyclodextrin, followed by dialysis, to homogeneity
recombinant enzyme from Escherichia coli strain MV1184 by heat treatment at 50°C for 30 min, two steps of ion exchange chromatography, and gel filtration
-
recombinant enzyme from Escherichia coli strain MV1184 by heat treatment at 56°C for 30 min, two steps of ion exchange chromatography, and gel filtration
-
recombinant enzyme from Pichia pastoris by ultrafiltration, gel filtration, and anion exchange chromatography to homogeneity
recombinant enzyme from Pichia pastoris strain GS115 culture supernatant
Thermochaetoides thermophila
recombinant enzyme mutant S54P/T314A/H415Y from Saccharomyces cerevisiae by acarbose affinity chromatography
-
recombinant enzyme to homogeneity from Saccharomyces cerevisiae cell culture medium, by ammonium sulfate fractionation, and anion exchange and affinity chromatography
-
recombinant glucoamylase
-
recombinant glucoamylase subunit Ct-MGAM splice form N20 and subunit Ct-SI from Spodoptera frugiperda Sf9 cells
recombinant glucoamylase, acarbose affinity chromatography
recombinant glucoamylase, ultrafiltration, SP-Sepharose, Q-Sepharose
recombinant His-tagged enzyme 10fold from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged enzyme 50.2fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant intracellular enzyme 37fold from Escherichia coli by heat treatment at 70°C for 30 min, anion exchange chromatography, and gel filtration to homogeneity
recombinant secreted His-tagged N-terminal subunit of human small intestinal enzyme from S2 cell culture medium by nickel affinity and anion exchange chromatography, followed by dialysis
-
recombinant wild-type and mutant enzymes from Saccharomyces cerevisiae strain AH22 medium by gel filtration and ion exchange chromatography to homogeneity
secreted, recombinant N-terminal catalytic domain from Drosophila S2 cell culture supernatant by chelating resin and anion exchange chromatography
-
starch affinity chromatography, partially purified
-
Superdex 200, Superose 12
three enzyme forms: glucoamylase 1, 2 and 3
-
three enzyme forms: I, II, and III
-
two enzyme forms: GA-I and GA-II
-
two native isozymes GA-I and GA-II, 5.8 and 9.6fold, respectively, to homogeneity
-
ultracentrifugation, anion exchange, gel permeation
-
ultrafiltrate, DEAE-Toyopearl 650 S, Sephadex G-150
-
ultrafiltration and gel filtration, anion exchange hydrophobic interaction chromatography
-
ultrafiltration, anion exchange, gel filtration
Endomycopsis fibuligera
-
ultrafiltration, gel filtration
-
ultrafiltration, gel filtration, affinity chromatography
-
ultrafiltration, ion exchange and gel filtration
-
wild-type enzyme
-