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3.2.1.23: beta-galactosidase

This is an abbreviated version!
For detailed information about beta-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.23

Reaction

beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose
+ 3 H2O = 4 beta-D-galactopyranose

Synonyms

Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, beta-D-galactoside galactohydrolase, beta-D-glactanase, beta-D-lactosidase, beta-gal, beta-Gal 1, beta-Gal 2, beta-Gal II, beta-galactosidase, beta-galactosidase I, beta-galactosidase II, beta-galactosidase III, beta-galactosidase IV, beta-galase, beta-lactosidase, betagal, betaGly4, betaGS, BGA, BgaB, BgaBM, bgaD, BgaH, BGAL, BGAL1, BGAL10, BGAL11, BGAL12, BGAL13, BGAL14, BGAL15, BGAL16, BGAL17, BGAL2, BGAL3, BGAL4, BGAL5, BGAL6, BGAL7, BGAL8, BGAL9, BgalA, BgAP, BgaS, BgaX, BGL1, BglAp, BGT I, BGT II, cold-active beta-galactosidase, CTP-beta-gal, driselase, EABase, endo-beta-galactosidase, Exo-(1-->4)-beta-D-galactanase, exo-beta-(1->3)-D-galactanase, Gal-2, Gal-5, GAL1, Gal2, Gal3, Gal4, GALA, galactanase, galactosidase, galactosidase, beta, GALB, gherkin lactase, H-BgaS, hcbetagal, Hlac_2868, Hydrolact, LacA, LACS, lactase, lactase phlorizin hydrolase, lactosylceramidase II, Lactozym, Lactozym 3000L, LPH, Maxilact, Maxilact-L/2000, MeBglD2, More, ONPGase, Oryzatym, p-nitrophenyl beta-galactosidase, PF1208, PRGH1, S 2107, SA-beta-GAL, SA-betagal, senescence-associated beta-galactosidase, SPD_0065 protein, SR12 protein, Ss beta-gal, SSO3019, SSU0587, Sumiklat, Trilactase, XC1214, XG-specific beta-galactosidase, YesZ, YH4502, ZD410

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.23 beta-galactosidase

Temperature Range

Temperature Range on EC 3.2.1.23 - beta-galactosidase

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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 10
high activity at 0°C
0 - 20
0 - 30
0°C: about 50% of maximal activity, 30°C: about 60% of maximal activity
0 - 45
-
the enzyme displays 52-85% maximum activity at 0-20°C, about 50% activity is seen at 45°C
10 - 40
10°C: 20% of maximal activity, 40°C: optimum
20 - 30
-
optimal activity
20 - 40
-
20°C: about 65% of maximal activity, 35°C: about 85% of maximal activity, 40°C: about 25% of maximal activity
20 - 55
-
20°C: about 40% of maximal activity, 55°C: about 50% of maximal activity, hydrolysis of lactose, strain L103
20 - 60
20 - 65
-
-
22 - 50
temperature profile, about 50% of maximal activity between 20°C and 45°C
24 - 56
24°C: about 45% of maximal activity, 56°C: about 70% of maximal activity
28 - 48
28°C: about 45% of maximal activity, 48°C: optimum, 50°C: about 5% of maximal activity, hydrolysis of o-nitrophenyl beta-D-galactopyranoside
30 - 50
30 - 55
-
30°C: about 45% of maximal activity, 55°C: about 25% of maximal activity
30 - 60
-
30°C: about 40% of maximal activity, 60°C: about 60% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L103
30 - 70
-
30°C: about 40% of maximal activity, 70°C: about 30% of maximal activity
35 - 50
Bacteroides polypragmatus
-
35°C: 70% of maximal activity, 50°C: 20% of maximal activity
35 - 60
35 - 80
-
35°C: about 40% of maximal activity, 80°C: about 20% of maximal activity
35 - 95
-
temperature profile, 3% of maximal activity at 30°C
37 - 80
the enzyme retains more than 40% of maximum activity between 37 and 80°C
4
10% of maximaln activity
4 - 50
-
immobilization of the recombinant Escherichia coli in alginate beads clearly enhances the enzyme activity at various temperatures, including 4 and 50°C, and at pH values from 4.0 to 8.5
40 - 60
40 - 65
40 - 70
-
about 45% of maximal activity at 40°C and at 70°C
40 - 80
Thermoanaerobium sp.
-
40°C: 50% of maximal activity, 80°C: optimum
50 - 70
-
50°C: about 45% of maximal activity, 70°C: about 30% of maximal activity
55 - 70
-
at 55°C half-maximum activity, at 70°C 80% enzyme activity
60 - 100
60°C: about 50% of maximal activity, 100°C: about 95% of maximal activity
60 - 65
-
temperature optimum for galactose hydrolysis
60 - 85
-
60°C: about 75% of maximal activity, 85°C: about 90% of maximal activity
65 - 85
-
at 65°C and 85°C the activity is approximately 60% of the maximum
65 - 95
65°C; about 55% of maximal activity, 95°C: about 90% of maximal activity, recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl-beta-D-galactopyranoside
70 - 95
70°C: about 60% of maximal activity, 95°C: about 70% of maximal activity
75
-
80% of maximum activity
80 - 90
substrate: 2-nitrophenyl beta-D-galactopyranoside, mutant enzymes F441Y and F359Q show 80% of their maximal activity
93 - 110
93°C: maximal activity, 110°C: active at temperatures up to
additional information