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3.2.1.23: beta-galactosidase

This is an abbreviated version!
For detailed information about beta-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.23

Reaction

beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose
+ 3 H2O = 4 beta-D-galactopyranose

Synonyms

Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, beta-D-galactoside galactohydrolase, beta-D-glactanase, beta-D-lactosidase, beta-gal, beta-Gal 1, beta-Gal 2, beta-Gal II, beta-galactosidase, beta-galactosidase I, beta-galactosidase II, beta-galactosidase III, beta-galactosidase IV, beta-galase, beta-lactosidase, betagal, betaGly4, betaGS, BGA, BgaB, BgaBM, bgaD, BgaH, BGAL, BGAL1, BGAL10, BGAL11, BGAL12, BGAL13, BGAL14, BGAL15, BGAL16, BGAL17, BGAL2, BGAL3, BGAL4, BGAL5, BGAL6, BGAL7, BGAL8, BGAL9, BgalA, BgAP, BgaS, BgaX, BGL1, BglAp, BGT I, BGT II, cold-active beta-galactosidase, CTP-beta-gal, driselase, EABase, endo-beta-galactosidase, Exo-(1-->4)-beta-D-galactanase, exo-beta-(1->3)-D-galactanase, Gal-2, Gal-5, GAL1, Gal2, Gal3, Gal4, GALA, galactanase, galactosidase, galactosidase, beta, GALB, gherkin lactase, H-BgaS, hcbetagal, Hlac_2868, Hydrolact, LacA, LACS, lactase, lactase phlorizin hydrolase, lactosylceramidase II, Lactozym, Lactozym 3000L, LPH, Maxilact, Maxilact-L/2000, MeBglD2, More, ONPGase, Oryzatym, p-nitrophenyl beta-galactosidase, PF1208, PRGH1, S 2107, SA-beta-GAL, SA-betagal, senescence-associated beta-galactosidase, SPD_0065 protein, SR12 protein, Ss beta-gal, SSO3019, SSU0587, Sumiklat, Trilactase, XC1214, XG-specific beta-galactosidase, YesZ, YH4502, ZD410

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.23 beta-galactosidase

Purification

Purification on EC 3.2.1.23 - beta-galactosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 enzyme forms
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4 isoenzymes: betaG630, betaG550, betaG41 and betaG19
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84000 Da precursor expressed in recombinant baculovirus-infected Spodoptera frugiperda
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a 84000 Da enzyme form and a 97000 Da enzyme form
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a method for the release of beta-D-galactosidase from yeast cells, particularly suited to industrial-scale enzyme recoveries
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a single step concanvalin A affinity chromatography for native PRGH1 improves the yield and reduces the purification time
acid neuraminidase and beta-galactosidase are present as an enzyme complex
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ammonium sulfate precipitation and fast-performance liquid chromatography using an anion-exchange column equilibrated with 100 mM sodium acetate buffer (pH 5.5)
ammonium sulfate precipitation and p-aminobenzyl-1-thio-beta-D-galactopyranoside-agarose column chromatography
ammonium sulfate precipitation and Sephadex G-100 gel filtration
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ammonium sulfate precipitation, followed by desalting, and sequentially applied to a DEAE Sepharose Fast Flow column, a Gigapite K-100 S column and a para-aminophenyl-1-thio-beta-D-galactopyranoside (APTG) affinity chromatography column
ammonium sulfate precipitation, octyl Sepharose column chromatography, and DEAE-Sephacel gel filtration
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ammonium sulfate precipitation, phenyl-Sepharose column chromatography
anti-FLAG M2 affinity gel filtration
beta-D-galactoside galactohydrolase pH 4.2, beta-D-galactoside galactohydrolase pH 4.5, beta-D-galactoside galactohydrolase pH 7.5
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beta-galactosidase I
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DEAE-Sepharose column chromatography, PABTG-Sepharose column chromatography, and Sephacryl S-300 gel filtration
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DEAE-Sepharose column chromatography, Sephacryl S200 gel filtration, phenyl-agarose column chromatography, and PBE 94 chromatofocusing column chromatography
enzyme form A1, A2, and B1
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enzyme from cells grown at pH 7.6 and enzyme from cells grown at pH 10.2
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enzyme from mutant strain H26-10-7 and from wild-type strain H26, 15.3fold and 12.8fold, respectively, by metal affinity chromatography and gel filtration
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gene fusion between galactose dehydrogenase, beta-galactosidase and galactokinase
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His-Trap column chromatography
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HisTrap column chromatography and MonoQ column chromatography
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Imac resin column chromatography
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multiple forms
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native enzyme 115fold to homogeneity by gel filtration, and anion exchange and hydroxylapatite chromatography
native enzyme 135fold by ammonium sulfate fractionation, concanavalin A affinity chromatography, anion and cation exchange chromatography, and gel filtration to homogeneity
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native enzyme 15.7fold by anion exchange chromatography and gel filtration
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native enzyme 163fold to homogeneity by ammonium sulfate, gel filtration, anion exchange chromatography, ultrafiltration, affinity chromatography, and preparative electrophoresis
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native enzyme 28fold to homogeneity by ammonium sulfate fractionation, and hydrophobic interaction and 4-aminobenzyl thiogalactoside affinity chromatography
native enzyme 46fold by anion exchange and hydrophobic interaction chromatography and gel filtration, recombinant GST-tagged enzyme from Escherichia coli strain BL21RB791 by heat treatment at 60°C and hydrophobic interaction chromatography to over 95% purity
native enzyme by ion exchange chromatography and gel filtration to homogeneity
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native enzyme by MnSO4 or streptomycin precipitation, ammonium sulfate fractionation, and starch column electrophoresis
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native enzyme to homogeneity by 4-aminophenyl beta-D-thiogalactopyranoside affinity chromatography
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native isozymes BGT I and BGT II by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, isozyme BGT I id purified 13fold, and isozyme BGT II 8.4fold
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native xyloglucan-specific enzyme 13.5fold from cotyledon of developing seedlings by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
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neutral beta-galactosidase
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Ni2+-NTA agarose resin column chromatography
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one-step purification
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p-aminobenzyl 1-thio-beta-D-galactopyranoside column chromatography
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partial
ProBond Ni column chromatography
Q sepharose column chromatography
recombinant enzyme
recombinant enzyme from Escherichia coli partially by ammonium sulfate fractionation and anion exchange chromatography
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recombinant enzyme from Escherichia coli strain JM109
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recombinant His-tagged cold-active enzyme 47.4fold from Escherichia coli by nickel affinity chromatography
recombinant His-tagged LacA from Escherichia coli by nickel affinity chromatography
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recombinant His6-tagged enzyme
recombinant mutant enzymes from Escherichia coli by ammonium sulfate fractionation and hydrophobic interaction chromatography
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recombinant OsBGal1 thioredoxin fusion protein 23fold from Escherichia coli by nickel affinity and anion exchange chromatography
recombinant soluble enzyme partially from Saccharomyces cerevisiae by heat treatment at 75°C for 30 min, and ultrafiltration
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soluble, native enzyme 966fold by anion exchange chromatography, precipitation at pH 4.5, dialysis, 4-aminophenyl-beta-D-thiogalactopyranoside affinity chromatography, and another step of anion exchange chromatography to homogeneity
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Superose 6 gel filtration and Superose 12 gel filtration
the mature enzyme is purified by DEAE-cellulose column chromatography and Sephacryl S-300 gel filtration, the His-tagged enzyme is purified by Bio-Scale Mini Profinity immobilized metal affinity chromatography
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use of the reverse micellar system of sodium bis(2-ethylhexyl)sulfosuccinate/isooctane from the aqueous extract of barley. The extent of reverse micellar solubilization of proteins increases with an increase in protein concentration of the feed sample. beta-Galactosidase is extracted with an activity recovery of 98.74% and a degree of purification of 7.2fold
using (NH4)2SO4 fractionation and affinity chromatography
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using gel filtration and anion-exchange chromatography
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using Ni-NTA chromatography