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3.2.1.23: beta-galactosidase

This is an abbreviated version!
For detailed information about beta-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.23

Reaction

beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose
+ 3 H2O = 4 beta-D-galactopyranose

Synonyms

Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, beta-D-galactoside galactohydrolase, beta-D-glactanase, beta-D-lactosidase, beta-gal, beta-Gal 1, beta-Gal 2, beta-Gal II, beta-galactosidase, beta-galactosidase I, beta-galactosidase II, beta-galactosidase III, beta-galactosidase IV, beta-galase, beta-lactosidase, betagal, betaGly4, betaGS, BGA, BgaB, BgaBM, bgaD, BgaH, BGAL, BGAL1, BGAL10, BGAL11, BGAL12, BGAL13, BGAL14, BGAL15, BGAL16, BGAL17, BGAL2, BGAL3, BGAL4, BGAL5, BGAL6, BGAL7, BGAL8, BGAL9, BgalA, BgAP, BgaS, BgaX, BGL1, BglAp, BGT I, BGT II, cold-active beta-galactosidase, CTP-beta-gal, driselase, EABase, endo-beta-galactosidase, Exo-(1-->4)-beta-D-galactanase, exo-beta-(1->3)-D-galactanase, Gal-2, Gal-5, GAL1, Gal2, Gal3, Gal4, GALA, galactanase, galactosidase, galactosidase, beta, GALB, gherkin lactase, H-BgaS, hcbetagal, Hlac_2868, Hydrolact, LacA, LACS, lactase, lactase phlorizin hydrolase, lactosylceramidase II, Lactozym, Lactozym 3000L, LPH, Maxilact, Maxilact-L/2000, MeBglD2, More, ONPGase, Oryzatym, p-nitrophenyl beta-galactosidase, PF1208, PRGH1, S 2107, SA-beta-GAL, SA-betagal, senescence-associated beta-galactosidase, SPD_0065 protein, SR12 protein, Ss beta-gal, SSO3019, SSU0587, Sumiklat, Trilactase, XC1214, XG-specific beta-galactosidase, YesZ, YH4502, ZD410

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.23 beta-galactosidase

Cloned

Cloned on EC 3.2.1.23 - beta-galactosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
84000 Da precursor expressed in recombinant baculovirus-infected Spodoptera frugiperda
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cloned in pET15b vector, overexpression in Escherichia coli BL21 (DE3) pLysS
cloned into the pET-28b vector. His-tagged MeBglD2 is expressed in Escherichia coli BL21(DE3) cells
ColR and oprB1 knockouts are constructed and expressed in Escherichia coli
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DNA and amino acid sequence determination and analysis, expression in Escherichia coli, enzyme activity is only detected when translation is initiated at the ATG at position 118
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expressed as His-tagged enzyme in Escherichia coli MC4100 cells
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expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli ER2566
expressed in Escherichia coli ER2566 cells
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expressed in Escherichia coli JM109 cells
expressed in Escherichia coli MRS32 cells
expressed in Escherichia coli TOP10F' cells and Pichia pastoris strain GS115
expressed in Escherichia coli TOP10F‘ cells
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expressed in Halobacterium sp. NRC-1 under the control of a cold shock protein (cspD2) gene promoter. The enzyme is produced at 20fold higher levels compared to Halorubrum lacusprofundi
expressed in Pichia pastoris as a His-tagged fusion protein
expressed in Pichia pastoris GS115 cells
expressed in Saccharomyces cerevisiae BJ3505 cells
expressed in the genetically tractable haloarchaeon Halobacterium sp. NRC-1 under the control of a cold shock protein (cspD2) gene promoter
expression in Bacillus subtilis
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expression in Bacillus subtilis WB600
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expression in CHO-K1 cell
expression in COS cells
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expression in Escherichia coli
expression in Escherichia coli as a soluble His-tagged recombinant enzyme
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expression in Escherichia coli as a soluble protein that contained a hexa-histidine tag at the carboxyl terminus
expression in Escherichia coli BL21. The active inclusion bodies of beta-galactosidase are easily isolated by nonionic detergent treatment and directly used for lactose conversion in a repetitive batch mode
expression in Escherichia coli strain JM109
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expression in Escherichia coli under control of araBD promoter
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expression in Escherichia coli, complementation of a lactose-negative Escherichia coli mutant
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expression in Escherichia coli, with and without His-tag
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expression in Halobacterium salinarum
expression in Lactobacillus plantarum WCFS1
expression in Pichia pastoris
expression in simian CV1 and murine NIH3T3 cell lines. The recombinant protein has no cytotoxic effect on the host cells
expression of His6-tagged BgaS enzyme and mutant variants in Escherichia coli strain MC1061 (DE3)
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expression of mutant enzymes in Escherichia coli
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expression of wild-type and recombinant hybrid mutant enzymes in Escherichia coli strain DH5alpha
gene bglA, overexpression of His-tagged cold-active beta-galactosidase in Escherichia coli strain DH5alpha
gene hlacz-sh, chemical synthesis of the gene derived from Pyrococcus woesei for direct evolution, DNA and amino acid sequence determination and anaylsis, library construction, expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta-Blue (DE3)
gene lacB, construction of a genomic library, DNA and amino acid sequence determination and anaylsis, expression as GST-tagged enzyme in Escherichia coli strain BL21RB791
gene lacL encoding the large subunits of the enzyme, DNA and amino acid sequence determinatin and anaylsis
gene lacM encoding the small subunits of the enzyme, DNA and amino acid sequence determinatin and anaylsis
gene lacS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
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gene lacS, expression in Saccharomyces cerevisiae strain ScKY117 using a galactose-inducible promoter, the strong, heat-inducible upstream activating RcC9 sequence of the yeast genes GAL1 and GAL10, originally isolated from Caenorhabditis elegans, transcription of the lacS gene under the control of this sequence is rapidly and efficiently induced by heat shock, the recombinant enzyme is identical in molecular mass, thermostability, and thermophilicity to the native enzyme, heat induction by growth at 39°C for 45 min, overview
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gene mbgA, DNA and amino acid sequence determination and anaylsis, genetic organization, a 27-bp inverted repeat overlaps the mbgA promoter sequence, and is a target for catabolite repressors, expression in Escherichia coli strain JM109
genes yesZ, lacA and galA, genetic organization, library construction and screening for 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside hydrolyzing activity, expression of His-tagged enzymes in Escherichia coli, cells expressing LacA with GalA gain the ability to use galactan as a carbon source
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isozymes OsBGal1 and OsBGal2, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional expression of soluble isozymes in Escherichia coli, expression of isozyme OsBGal1 thioredoxin fusion protein
mutant F441Y is expressed in Pichia pastoris
overexpression in Escherichia coli
overexpression in Sulfolobus solfataricus mutant PBL2025, that misses 50 genes (SSO3004-3050), including genes coding for a multitude of enzymes possibly involved in sugar degradation or metabolism
poly-His-tagged beta-galactosidase overexpressed in Escherichia coli
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quantitative expression analysis
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the bgaH gene is engineered into a halobacterial plasmid vector and introduced into Haloferax volcanii, transformants are shown to express the enzyme
transfection of fibroblast
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