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3.2.1.23: beta-galactosidase

This is an abbreviated version!
For detailed information about beta-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.23

Reaction

beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose
+ 3 H2O = 4 beta-D-galactopyranose

Synonyms

Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, beta-D-galactoside galactohydrolase, beta-D-glactanase, beta-D-lactosidase, beta-gal, beta-Gal 1, beta-Gal 2, beta-Gal II, beta-galactosidase, beta-galactosidase I, beta-galactosidase II, beta-galactosidase III, beta-galactosidase IV, beta-galase, beta-lactosidase, betagal, betaGly4, betaGS, BGA, BgaB, BgaBM, bgaD, BgaH, BGAL, BGAL1, BGAL10, BGAL11, BGAL12, BGAL13, BGAL14, BGAL15, BGAL16, BGAL17, BGAL2, BGAL3, BGAL4, BGAL5, BGAL6, BGAL7, BGAL8, BGAL9, BgalA, BgAP, BgaS, BgaX, BGL1, BglAp, BGT I, BGT II, cold-active beta-galactosidase, CTP-beta-gal, driselase, EABase, endo-beta-galactosidase, Exo-(1-->4)-beta-D-galactanase, exo-beta-(1->3)-D-galactanase, Gal-2, Gal-5, GAL1, Gal2, Gal3, Gal4, GALA, galactanase, galactosidase, galactosidase, beta, GALB, gherkin lactase, H-BgaS, hcbetagal, Hlac_2868, Hydrolact, LacA, LACS, lactase, lactase phlorizin hydrolase, lactosylceramidase II, Lactozym, Lactozym 3000L, LPH, Maxilact, Maxilact-L/2000, MeBglD2, More, ONPGase, Oryzatym, p-nitrophenyl beta-galactosidase, PF1208, PRGH1, S 2107, SA-beta-GAL, SA-betagal, senescence-associated beta-galactosidase, SPD_0065 protein, SR12 protein, Ss beta-gal, SSO3019, SSU0587, Sumiklat, Trilactase, XC1214, XG-specific beta-galactosidase, YesZ, YH4502, ZD410

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.23 beta-galactosidase

pH Range

pH Range on EC 3.2.1.23 - beta-galactosidase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.5 - 8
purified recombinant BgalA has measurable enzymatic activity over a pH range of 0.5 through 8.0 (measured at 37°C)
2.2 - 4.6
-
about 50% of maximal activity at pH 2.2 and at pH 4.6
2.3 - 6
-
pH 2.3: about 30% of maximal activity, pH 6.0: about 50% of maximal activity
2.5 - 5
inactivation at pH 2.5, half-maximal activity at pH 4.5-5.0, recombinant isozyme OsGal1
2.5 - 6
-
pH 2.5: about 50% of maximal activity, pH 6.0: about 45% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside
3 - 6
-
pH 2.0: less than 10% of maximal activity, pH 3.0: optimum, pH 5.0: about 80% of maximal activity, pH 6.0: about 30% of maximal activity
3 - 6.5
-
pH 3.0: about 45% of maximal activity, pH 6.5: about 40% of maximal activity, hydrolysis of lactose
3 - 7
-
pH 3.0: about 90% of maximal activity, pH 7.0: about 40% of maximal activity
3.2 - 6.2
-
pH 3.2: about 90% of maximal activity, pH 6.2: about 45% of maximal activity
3.5 - 10
3.5 - 6.5
-
pH 3.0: about 15% of maximal activity, pH 3.5: about 85% of maximal activity, pH 6.5: about 50% of maximal activity, pH 7.0: about 35% of maximal activity
3.8 - 5
Kerstingiella geocarpa
-
pH 3.8: about 50% of maximal activity, pH 5.0: about 55% of maximal activity
4 - 6.5
-
about 70% of maximal activity at pH 4.0 and at 6.5
4 - 8
4 - 8.5
-
immobilization of the recombinant Escherichia coli in alginate beads clearly enhances the enzyme activity at various temperatures, including 4°C and 50°C, and at pH values from 4.0 to 8.5
4 - 9
-
active in this range
4.5 - 6.5
-
about 45% of maximal activity at pH 4.5 and at pH 6.5
4.5 - 7.5
pH 4.5: about 85% of maximal activity, pH 7.5: about 50% of maximal activity, recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl-beta-D-galactopyranoside
5 - 11
-
60% of maximal activity at pH 7.5 and pH 9.0
5 - 6
wild-type enzyme and mutant enzymes F441Y and F359Q
5 - 7
5 - 7.5
-
pH 5.0: about 65% of maximal activity, pH 7.5: about 50% of maximal activity
5.2 - 7.6
-
more than 50% of maximal activity at pH 5.2 and at pH 7.6
5.4 - 7.8
Bacteroides polypragmatus
-
pH 5.4: 7.4% of maximal activity, pH 7.8: 16.2% of maximal activity
5.5 - 7.5
5.5 - 7.7
-
pH 5.5: about 50% of maximal activity, pH 7.7: about 30% of maximal activity
5.5 - 8.8
-
pH 5.5: about 50% of maximal activity, pH 8.8: about 60% of maximal activity, hydrolysis of lactose, strain L461
6 - 7.5
6 - 8
-
80% activity
6 - 8.5
-
pH 6.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L103
6 - 9
6.2 - 8.5
-
pH 6.2: about 60% of maximal activity, pH 8.5: about 75% of maximal activity, hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L461
6.5 - 7.5
6.5 - 8.5
over 90% of the maximum activity in the pH range of 6.5-8.5
7 - 9
over 80% of maximal activity within this range
additional information