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Literature summary for 3.2.1.23 extracted from

  • Chen, W.; Chen, H.; Xia, Y.; Zhao, J.; Tian, F.; Zhang, H.
    Production, purification, and characterization of a potential thermostable galactosidase for milk lactose hydrolysis from Bacillus stearothermophilus (2008), J. Dairy Sci., 91, 1751-1758.
    View publication on PubMed

Application

Application Comment Organism
nutrition dairy processing Geobacillus stearothermophilus
nutrition enzyme is suitable for both the hydrolysis of lactose and the production of galacto-oligosaccharides in milk processing Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Geobacillus stearothermophilus
expression in Bacillus subtilis WB600 Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, 85% inhibition Geobacillus stearothermophilus
D-galactose
-
Geobacillus stearothermophilus
Fe2+ 5 mM, 52% inhibition Geobacillus stearothermophilus
iodoacetic acid 1 mM, 77% inhibition; inhibition of 73.0% enzyme activity Geobacillus stearothermophilus
lactose
-
Geobacillus stearothermophilus
p-chloromercuribenzoate 1 mM, 85% inhibition Geobacillus stearothermophilus
p-chloromercuribenzoic acid inhibition of 86.2% enzyme activity Geobacillus stearothermophilus
Pb2+ 2 mM, complete inhibition; no enzyme activity Geobacillus stearothermophilus
Sn2+ no enzyme activity Geobacillus stearothermophilus
Zn2+ 5 mM, 76% inhibition Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.96
-
lactose
-
Geobacillus stearothermophilus
2.96
-
2-nitrophenyl-beta-D-galactopyranoside
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
SDS-PAGE Geobacillus stearothermophilus
78000
-
x * 70000, SDS-PAGE, x * 78000, calculated Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
isoform BgaB
-
Geobacillus stearothermophilus
-
strain ATCC8005
-
Geobacillus stearothermophilus ATCC 8005
-
strain ATCC8005
-

Purification (Commentary)

Purification (Comment) Organism
19.4fold Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5
-
cell-free extract Geobacillus stearothermophilus
125.2
-
-
Geobacillus stearothermophilus
125.2
-
after 19.4-fold purification Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O pH 5.0-9.0, 55°C Geobacillus stearothermophilus 2-nitrophenol + beta-D-galactose
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O pH 5.0-9.0, 55°C Geobacillus stearothermophilus ATCC 8005 2-nitrophenol + beta-D-galactose
-
?
lactose + H2O
-
Geobacillus stearothermophilus D-glucose + D-galactose
-
?
lactose + H2O
-
Geobacillus stearothermophilus ATCC 8005 D-glucose + D-galactose
-
?
additional information high level of transgalactosidase activity in hydrolysis of lactose in milk Geobacillus stearothermophilus ?
-
?
additional information high level of transgalactosidase activity in hydrolysis of lactose in milk Geobacillus stearothermophilus ATCC 8005 ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE, x * 78000, calculated Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
beta-D-galactohydrolase
-
Geobacillus stearothermophilus
beta-galactosidase
-
Geobacillus stearothermophilus
galactosidase
-
Geobacillus stearothermophilus
lactase
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Geobacillus stearothermophilus
70
-
pH 7.0, maximum activity Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 70 at 55°C half-maximum activity, at 70°C 80% enzyme activity Geobacillus stearothermophilus
75
-
80% of maximum activity Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
6 h, enzymatic half-life 120 h Geobacillus stearothermophilus
65
-
5 h, enzymatic half-life 50 h Geobacillus stearothermophilus
65
-
half-life 50 h Geobacillus stearothermophilus
70
-
4 h, enzymatic half-life 9 h Geobacillus stearothermophilus
70
-
half-life 9 h Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Geobacillus stearothermophilus
7
-
70°C, maximum activity Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 80% enzyme activity Geobacillus stearothermophilus
6.5 7.5 more than 80% of maximum activity Geobacillus stearothermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
15.7
-
lactose
-
Geobacillus stearothermophilus
87.3
-
D-galactose
-
Geobacillus stearothermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus stearothermophilus isoelectric focusing
-
5.1
Geobacillus stearothermophilus isoelectric focusing gel electrophoresis
-
5.1
Geobacillus stearothermophilus calculated
-
5.7