Application | Comment | Organism |
---|---|---|
nutrition | dairy processing | Geobacillus stearothermophilus |
nutrition | enzyme is suitable for both the hydrolysis of lactose and the production of galacto-oligosaccharides in milk processing | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Geobacillus stearothermophilus |
expression in Bacillus subtilis WB600 | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 85% inhibition | Geobacillus stearothermophilus | |
D-galactose | - |
Geobacillus stearothermophilus | |
Fe2+ | 5 mM, 52% inhibition | Geobacillus stearothermophilus | |
iodoacetic acid | 1 mM, 77% inhibition; inhibition of 73.0% enzyme activity | Geobacillus stearothermophilus | |
lactose | - |
Geobacillus stearothermophilus | |
p-chloromercuribenzoate | 1 mM, 85% inhibition | Geobacillus stearothermophilus | |
p-chloromercuribenzoic acid | inhibition of 86.2% enzyme activity | Geobacillus stearothermophilus | |
Pb2+ | 2 mM, complete inhibition; no enzyme activity | Geobacillus stearothermophilus | |
Sn2+ | no enzyme activity | Geobacillus stearothermophilus | |
Zn2+ | 5 mM, 76% inhibition | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.96 | - |
lactose | - |
Geobacillus stearothermophilus | |
2.96 | - |
2-nitrophenyl-beta-D-galactopyranoside | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
78000 | - |
x * 70000, SDS-PAGE, x * 78000, calculated | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
isoform BgaB | - |
Geobacillus stearothermophilus | - |
strain ATCC8005 | - |
Geobacillus stearothermophilus ATCC 8005 | - |
strain ATCC8005 | - |
Purification (Comment) | Organism |
---|---|
19.4fold | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.5 | - |
cell-free extract | Geobacillus stearothermophilus |
125.2 | - |
- |
Geobacillus stearothermophilus |
125.2 | - |
after 19.4-fold purification | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | pH 5.0-9.0, 55°C | Geobacillus stearothermophilus | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | pH 5.0-9.0, 55°C | Geobacillus stearothermophilus ATCC 8005 | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Geobacillus stearothermophilus | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Geobacillus stearothermophilus ATCC 8005 | D-glucose + D-galactose | - |
? | |
additional information | high level of transgalactosidase activity in hydrolysis of lactose in milk | Geobacillus stearothermophilus | ? | - |
? | |
additional information | high level of transgalactosidase activity in hydrolysis of lactose in milk | Geobacillus stearothermophilus ATCC 8005 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE, x * 78000, calculated | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
beta-D-galactohydrolase | - |
Geobacillus stearothermophilus |
beta-galactosidase | - |
Geobacillus stearothermophilus |
galactosidase | - |
Geobacillus stearothermophilus |
lactase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus stearothermophilus |
70 | - |
pH 7.0, maximum activity | Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 70 | at 55°C half-maximum activity, at 70°C 80% enzyme activity | Geobacillus stearothermophilus |
75 | - |
80% of maximum activity | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
6 h, enzymatic half-life 120 h | Geobacillus stearothermophilus |
65 | - |
5 h, enzymatic half-life 50 h | Geobacillus stearothermophilus |
65 | - |
half-life 50 h | Geobacillus stearothermophilus |
70 | - |
4 h, enzymatic half-life 9 h | Geobacillus stearothermophilus |
70 | - |
half-life 9 h | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Geobacillus stearothermophilus |
7 | - |
70°C, maximum activity | Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | 80% enzyme activity | Geobacillus stearothermophilus |
6.5 | 7.5 | more than 80% of maximum activity | Geobacillus stearothermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
15.7 | - |
lactose | - |
Geobacillus stearothermophilus | |
87.3 | - |
D-galactose | - |
Geobacillus stearothermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus stearothermophilus | isoelectric focusing | - |
5.1 |
Geobacillus stearothermophilus | isoelectric focusing gel electrophoresis | - |
5.1 |
Geobacillus stearothermophilus | calculated | - |
5.7 |