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Literature summary for 3.2.1.23 extracted from
- Purification and properties of recombinant beta-galactosidase from Bacillus circulans (1998), Glycoconj. J., 15, 155-160.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Niallia circulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.69 | - |
p-nitrophenyl-beta-D-galactoside | - |
Niallia circulans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
1 * 62000, SDS-PAGE | Niallia circulans |
| 67000 | - |
gel filtration | Niallia circulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Niallia circulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.13 | - |
- |
Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | transglycosylation | Niallia circulans | ? | - |
? | |
| additional information | high selectivity for oligosaccharides which have terminal beta1-3 linked galactose residues, no hydrolysis of beta1-4 linked galactooligosaccharides | Niallia circulans | ? | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Niallia circulans | p-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62000, SDS-PAGE | Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Niallia circulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | about 45% of maximal activity at 40°C and at 70°C | Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable below | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | about 60% of maximal activity at pH 4.0 and at pH 10.0 | Niallia circulans |
| 5 | 6 | - |
Niallia circulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | stable | Niallia circulans |
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