Any feedback?
Literature summary for 3.2.1.23 extracted from
- A novel acid-stable, acid-active beta-galactosidase potentially suited to the alleviation of lactose intolerance (2010), Appl. Microbiol. Biotechnol., 86, 517-524.
General Stability
| General Stability | Organism |
|---|---|
| the purified enzyme retains 68% residual activity after in vitro exposure to simulated gastric conditions (pepsin at pH 2.0) for 2 h | Aspergillus niger |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 123000 | - |
glycosylated enzyme, gel filtration | Aspergillus niger |
| 129000 | - |
1 * 129000, glycosylated enzyme, SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | P29853 | strain DSM 22593 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography, Sephacryl S200 gel filtration, phenyl-agarose column chromatography, and PBE 94 chromatofocusing column chromatography | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
crude enzyme, in 200 mM potassium phosphate buffer, pH 6.8, for 15 min at 37°C | Aspergillus niger |
| 69.3 | - |
after 53.3fold purification, in 200 mM potassium phosphate buffer, pH 6.8, for 15 min at 37°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | 6% activity compared to 4-nitrophenyl alpha-D-galactopyranoside | Aspergillus niger | 2-nitrophenol + beta-D-galactopyranose | - |
? |  |
| 4-nitrophenyl alpha-D-galactopyranoside + H2O | 100% activity | Aspergillus niger | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinopyranoside + H2O | 42% activity compared to 4-nitrophenyl alpha-D-galactopyranoside | Aspergillus niger | 4-nitrophenol + alpha-L-arabinopyranose | - |
? | |
| 4-nitrophenyl beta-D-fucopyranoside + H2O | 3% activity compared to 4-nitrophenyl alpha-D-galactopyranoside | Aspergillus niger | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | 81% activity compared to 4-nitrophenyl alpha-D-galactopyranoside | Aspergillus niger | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O | - |
Aspergillus niger | 5-bromo-4-chloroindol + beta-D-galactose | - |
? | |
| lactose + H2O | - |
Aspergillus niger | D-galactose + D-glucose | - |
? | |
| additional information | no activity towards 4-nitrophenyl alpha-D-glucopyranoside and 4-nitrophenyl alpha-D-mannopyranoside | Aspergillus niger | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 129000, glycosylated enzyme, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lactase | - |
Aspergillus niger |
| lactase phlorizin hydrolase | - |
Aspergillus niger |
| LPH | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 75 | - |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | a sharp drop of activity occurs above 60°C, no activity is detected at 80°C | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 4 | - |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | a sharp drop of activity occurs above pH 4.0, about 35% residual activity at pH 4.5-5.5, about 10% residual activity at pH 6.5, no activity is detected at pH 8.0 and above | Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | glycosylated enzyme, isoelectric focusing | - |
4.7 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
