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Literature summary for 3.2.1.23 extracted from

  • Karan, R.; Capes, M.D.; DasSarma, P.; DasSarma, S.
    Cloning, overexpression, purification, and characterization of a polyextremophilic beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi (2013), BMC Biotechnol., 13, 3.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in the genetically tractable haloarchaeon Halobacterium sp. NRC-1 under the control of a cold shock protein (cspD2) gene promoter Halorubrum lacusprofundi

Inhibitors

Inhibitors Comment Organism Structure
ethanol 10% v/v, about 40% loss of activity Halorubrum lacusprofundi
isoamyl alcohol 10% v/v, about 35% loss of activity Halorubrum lacusprofundi
methanol 10% v/v, about 5% loss of activity Halorubrum lacusprofundi
n-butanol 10% v/v, about 35% loss of activity Halorubrum lacusprofundi

Metals/Ions

Metals/Ions Comment Organism Structure
KCl maximal activity in either 4 M NaCl or KCl Halorubrum lacusprofundi
NaCl maximal activity in either 4 M NaCl or KCl Halorubrum lacusprofundi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78060
-
 x * 78060, calculated from sequence Halorubrum lacusprofundi
100000
-
 x * 100000, SDS-PAGE Halorubrum lacusprofundi

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 10-20% v/v, 3–4% loss of activity after the first h and 25-30% after 3 hours Halorubrum lacusprofundi
isoamyl alcohol 10-20% v/v, 3–4% loss of activity after the first h and 25-30% after 3 hours Halorubrum lacusprofundi
Methanol 10-20% v/v, 3–4% loss of activity after the first h and 25-30% after 3 hours Halorubrum lacusprofundi
n-Butanol 10-20% v/v, 3–4% loss of activity after the first h and 25-30% after 3 hours Halorubrum lacusprofundi

Organism

Organism UniProt Comment Textmining
Halorubrum lacusprofundi B9LW38
-
-
Halorubrum lacusprofundi DSM 5036 B9LW38
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Halorubrum lacusprofundi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
110.8
-
 pH 6.0, 25°C Halorubrum lacusprofundi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Halorubrum lacusprofundi 2-nitrophenol + beta-D-galactose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Halorubrum lacusprofundi DSM 5036 2-nitrophenol + beta-D-galactose
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Halorubrum lacusprofundi
? x * 78060, calculated from sequence Halorubrum lacusprofundi

Synonyms

Synonyms Comment Organism
Hlac_2868 locus name Halorubrum lacusprofundi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Halorubrum lacusprofundi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 35°C: about 55% of maximal activity, 60°C: about 80% of maximal activity Halorubrum lacusprofundi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Halorubrum lacusprofundi

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 pH 6.0: about 60% of maximal activity, pH 7.5: about 40% of maximal activity Halorubrum lacusprofundi