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3.1.26.12: ribonuclease E

This is an abbreviated version!
For detailed information about ribonuclease E, go to the full flat file.

Word Map on EC 3.1.26.12

Reaction

endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions =

Synonyms

Ams/Rne/Hmp1 polypeptide, AqaRng, endoribonuclease E, endoribonuclease RNase E, More, NCgl2281, ribonuclease E, RNase E, RNase E/G, RNase E/G-type endoribonuclease, RNase ES, RNase EV, RNaseE, Rne, Rne protein, RneC, Rng, SSO1404, SynRne

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.12 ribonuclease E

Purification

Purification on EC 3.1.26.12 - ribonuclease E

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Toyopearl column filtration chromatography, and Affi-Gel blue column chromatography
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by affinity chromatography
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by ammonium sulfate precipitation and cation-exchange chromatography
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FLAG-tagged RNase E is purified by affinity chromatography
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immobilized metal affinity chromatography and Superdex 200 gel filtration
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N-terminal half-RNase E
native and recombinant ribonuclease E N-terminal catalytic domains from transformed Escherichia coli
native degradosomes from strain CF881, which lacks RNase I, further electrophoretically purification of Rne protein
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native RNA degradosome from strain BRL2288, recombinant His-tagged full-length wild-type RNase E and His-tagged N-terminal ribonucleolytic domain RTD-RNase E from strain BL21(DE3) by metal affinity chromatography
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Ni-NTA column chromatography
partially about 100fold by ammonium chloride precipitation, ultracentrifugation, ammonium sulfate fractionation, and gel filtration
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recombinant active His- and Myc-tagged truncated enzyme from strain BL21(DE3) by affinity chromatography
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recombinant C-terminally His6- and Myc-tagged N-terminal enzyme half and maltose-binding protein-fused N-terminal half from strain BL21(DE3) by affinity chromatography on a metal chelating resin and an amylose resin, respectively
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recombinant enzyme from Escherichia coli
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recombinant enzyme to near homogeneity by SDS-PAGE and renaturation
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recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
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recombinant His-tagged enzyme from strain BL21(DE3)
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recombinant His-tagged N-terminal catalytic domain from strain BL21(DE3) by nickel affinity chromatography and gel filtration to over 95% purity
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recombinant His-tagged N-terminal half of RNase E from strain BL21(DE3)
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recombinant His-tagged N-terminal RNaseE catalytic N domain from strain BL21(DE3) by metal affinity chromatography and gel filtration to homogeneity
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recombinant His-tagged truncated enzyme mutants from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis and gel filtration
recombinant His-tagged truncated enzyme mutants from strain BL21(DE3) by nickel affinity chromatography, dialysis and gel filtration
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recombinant His-tagged wild-type and mutant catalytic domains by nickel affinity chromatography
recombinant His10-tagged enzyme from Escherichia coli strain BL21(DE3) by His-trap chromatography with or without cleavage of the His-tag by factor Xa
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recombinant His10-tagged RNase E/G from Escherichia coli strain BL21(DE3) by metal affinity chromatography, removal of the His-tag by factorXa and dialysis
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recombinant isolated, His-tagged catalytic domain Rne498 from strain BL21(DE3) by metal affinity chromatography and dialysis to homogeneity
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recombinant N-terminally His6-tagged wild-type and mutant full-length enzymes, and isolated N-terminally His6-tagged S1 domain by nickel affinity chromatography, removal of the His-tags by thrombin
recombinant wild-type and mutant enzymes, purification involves denaturation and renaturation steps due to the poor solubility of the full-length enzyme
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recombinant wild-type enzyme and truncated mutant enzyme, partially
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