3.1.26.12: ribonuclease E
This is an abbreviated version!
For detailed information about ribonuclease E, go to the full flat file.
Word Map on EC 3.1.26.12
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3.1.26.12
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degradosome
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polynucleotide
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phosphorylase
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pnpase
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srnas
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endonuclease
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hfq
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helicase
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endonucleolytic
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exoribonuclease
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single-stranded
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enolase
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stem-loops
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polya
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rna-binding
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polycistronic
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ribonucleolytic
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e-dependent
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base-pairing
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dead-box
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autoregulation
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e-mediated
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e-like
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ompa
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endoribonucleolytic
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5'-terminal
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intercistronic
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cole1-type
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monophosphorylated
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5'-monophosphorylated
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shine-dalgarno
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rho-independent
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hfq-dependent
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glucosamine-6-phosphate
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riboswitches
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au-rich
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glm
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rna-processing
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crescentus
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analysis
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medicine
- 3.1.26.12
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degradosome
- polynucleotide
- phosphorylase
- pnpase
- srnas
- endonuclease
- hfq
- helicase
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endonucleolytic
- exoribonuclease
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single-stranded
- enolase
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stem-loops
- polya
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rna-binding
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polycistronic
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ribonucleolytic
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e-dependent
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base-pairing
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dead-box
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autoregulation
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e-mediated
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e-like
- ompa
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endoribonucleolytic
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5'-terminal
-
intercistronic
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cole1-type
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monophosphorylated
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5'-monophosphorylated
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shine-dalgarno
-
rho-independent
-
hfq-dependent
- glucosamine-6-phosphate
- riboswitches
-
au-rich
- glm
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rna-processing
- crescentus
- analysis
- medicine
Reaction
endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions =
Synonyms
Ams/Rne/Hmp1 polypeptide, AqaRng, endoribonuclease E, endoribonuclease RNase E, More, NCgl2281, ribonuclease E, RNase E, RNase E/G, RNase E/G-type endoribonuclease, RNase ES, RNase EV, RNaseE, Rne, Rne protein, RneC, Rng, SSO1404, SynRne
ECTree
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Purification
Purification on EC 3.1.26.12 - ribonuclease E
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ammonium sulfate precipitation, Toyopearl column filtration chromatography, and Affi-Gel blue column chromatography
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by ammonium sulfate precipitation and cation-exchange chromatography
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native and recombinant ribonuclease E N-terminal catalytic domains from transformed Escherichia coli
native degradosomes from strain CF881, which lacks RNase I, further electrophoretically purification of Rne protein
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native RNA degradosome from strain BRL2288, recombinant His-tagged full-length wild-type RNase E and His-tagged N-terminal ribonucleolytic domain RTD-RNase E from strain BL21(DE3) by metal affinity chromatography
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Ni-NTA column chromatography
partially about 100fold by ammonium chloride precipitation, ultracentrifugation, ammonium sulfate fractionation, and gel filtration
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recombinant active His- and Myc-tagged truncated enzyme from strain BL21(DE3) by affinity chromatography
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recombinant C-terminally His6- and Myc-tagged N-terminal enzyme half and maltose-binding protein-fused N-terminal half from strain BL21(DE3) by affinity chromatography on a metal chelating resin and an amylose resin, respectively
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recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
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recombinant His-tagged N-terminal catalytic domain from strain BL21(DE3) by nickel affinity chromatography and gel filtration to over 95% purity
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recombinant His-tagged N-terminal RNaseE catalytic N domain from strain BL21(DE3) by metal affinity chromatography and gel filtration to homogeneity
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recombinant His-tagged truncated enzyme mutants from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis and gel filtration
recombinant His-tagged truncated enzyme mutants from strain BL21(DE3) by nickel affinity chromatography, dialysis and gel filtration
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recombinant His-tagged wild-type and mutant catalytic domains by nickel affinity chromatography
recombinant His10-tagged enzyme from Escherichia coli strain BL21(DE3) by His-trap chromatography with or without cleavage of the His-tag by factor Xa
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recombinant His10-tagged RNase E/G from Escherichia coli strain BL21(DE3) by metal affinity chromatography, removal of the His-tag by factorXa and dialysis
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recombinant isolated, His-tagged catalytic domain Rne498 from strain BL21(DE3) by metal affinity chromatography and dialysis to homogeneity
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recombinant N-terminally His6-tagged wild-type and mutant full-length enzymes, and isolated N-terminally His6-tagged S1 domain by nickel affinity chromatography, removal of the His-tags by thrombin
recombinant wild-type and mutant enzymes, purification involves denaturation and renaturation steps due to the poor solubility of the full-length enzyme
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