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Literature summary for 3.1.26.12 extracted from
- Two distinct regions on the surface of an RNA-binding domain are crucial for RNase E function (2002), Mol. Microbiol., 46, 959-969.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F57A | site-directed mutagenesis, 91% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| F67A | site-directed mutagenesis, 94% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| K106A | site-directed mutagenesis, 60% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| K112A | site-directed mutagenesis, 94% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| K37A | site-directed mutagenesis, 94% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| K38A | site-directed mutagenesis, 49% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| K43A | site-directed mutagenesis, 33% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| K71A | site-directed mutagenesis, 56% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| R109A | site-directed mutagenesis, 78% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| R48A | site-directed mutagenesis, 49% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| R64A | site-directed mutagenesis, 77% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| R87A | site-directed mutagenesis, 16% increased feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| R95A | site-directed mutagenesis, 19% increased feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| Y42A | site-directed mutagenesis, 48% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| Y60A | site-directed mutagenesis, 99% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
| Y77A | site-directed mutagenesis, 19% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | the enzyme, especially its catalytic N-terminal domain, is essential for RNA processing and degradation, and for cell growth and feedback regulation of RNase E synthesis | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes, purification involves denaturation and renaturation steps due to the poor solubility of the full-length enzyme | Escherichia coli |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes, purification involves denaturation and renaturation steps due to the poor solubility of the full-length enzyme | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
feedback regulation activity of wild-type and mutant enzymes, overview | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | reaction mechanism | Escherichia coli | ? | - |
? | |
| additional information | the enzyme, especially its catalytic N-terminal domain, is essential for RNA processing and degradation, and for cell growth and feedback regulation of RNase E synthesis | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | three-dimensional enzyme structure with an essential N-terminal RNase E domain of the S1 family RNA-binding domain fold, the residues involved in cell growth and feedback regulation of RNase E synthesis form two cluster, e.g. Phe57, Phe67, and Tyr112, or Lys37 and Tyr60, overview, structural modeling of the S1 domain | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the S1 family of RNA-binding enzymes | Escherichia coli |
| RNase E | - |
Escherichia coli |
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