Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli GM402 BL21(DE3) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Toyopearl column filtration chromatography, and Affi-Gel blue column chromatography | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
the diluted Rne is dialyzed against two changes of 100 volumes each of 60 mM NH4Cl, 0.1 mM dithiothreitol, and 10% (v/v) glycerol at 4°C for at least 20 h | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the C-terminus of this enzyme serves as a scaffold to which other components of the RNA degradosome bind including the phosphorolytic 3'-exonuclease, polynucleotide phosphorylase, the DEAD-box RNA helicase RhlB, and the glycolytic enzyme enolase. The DEAD-box RNA helicases CsdA and RhlE and the RNA binding protein Hfq may bind to RNase E in place of one or more of the prototypical components. | Escherichia coli | ? | - |
? | |
additional information | the C-terminus of this enzyme serves as a scaffold to which other components of the RNA degradosome bind including the phosphorolytic 3'-exonuclease, polynucleotide phosphorylase, the DEAD-box RNA helicase RhlB, and the glycolytic enzyme enolase. The DEAD-box RNA helicases CsdA and RhlE and the RNA binding protein Hfq may bind to RNase E in place of one or more of the prototypical components. | Escherichia coli GM402 BL21(DE3) | ? | - |
? | |
RNA + H2O | - |
Escherichia coli | ? | - |
? | |
RNA + H2O | - |
Escherichia coli GM402 BL21(DE3) | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase E | - |
Escherichia coli |
Rne protein | - |
Escherichia coli |