Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | sequences sequestering the -71 site of bacteriophage T4 gene 32 mRNA inhibit the enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme assay in presence of Mg2+ and NH4Cl | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme plays an important role in the processing and degradation of bacteriophage T4 and Escherichia coli mRNAs, mutational processing site analysis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strains N3433 and N3431 | - |
Purification (Comment) | Organism |
---|---|
partially about 100fold by ammonium chloride precipitation, ultracentrifugation, ammonium sulfate fractionation, and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bacteriophage T4 gene 32 mRNA + H2O | processing at the -71 site, which forms a stem-loop essential for enzyme activity of RNase E, the putative consensus sequence is RAUUW, mutational disruption of the stem-loop leads to loss of activity, mechanism, overview | Escherichia coli | ? | - |
? | |
additional information | the enzyme plays an important role in the processing and degradation of bacteriophage T4 and Escherichia coli mRNAs, mutational processing site analysis, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endoribonuclease E | - |
Escherichia coli |
RNase E | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.9 | assay at | Escherichia coli |