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Literature summary for 3.1.26.12 extracted from
- Escherichia coli endoribonuclease RNase E: autoregulation of expression and site-specific cleavage of mRNA (1993), Mol. Microbiol., 9, 557-568.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in wild-type rne cells and in thermosensitive mutant rne-50 and rne-3071 cells, overexpression of truncated 110 kDa mutant enzyme in rne50 cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutation of gene rne affect the rate of mRNA decay in vivo, construction of a truncated 110 kDa mutant enzyme | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | enzyme assay in presence of 1 mM MnCl2 | Escherichia coli |  |
| NH4Cl | enzyme assay in presence of 0.1 M NH4Cl | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
x * 180000, unprocessed active enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ompA mRNA + H2O | Escherichia coli | ompA at a site which is rate determining for degradation and also cleaved by RNase K | ? | - |
? | |
| rne mRNA + H2O | Escherichia coli | the enzyme autoregulates its expression by cleavage and processing of its own rne mRNA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene rne or ams | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the recombinant enzyme is cleaved from an active 180 kDa form to active about 70 kDa and 60 kDa fragments during purification | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type enzyme and truncated mutant enzyme, partially | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme autoregulates its expression by cleavage and processing of its own rne mRNA | Escherichia coli | ? | - |
? | |
| ompA mRNA + H2O | processing | Escherichia coli | ? | - |
? | |
| ompA mRNA + H2O | ompA at a site which is rate determining for degradation and also cleaved by RNase K | Escherichia coli | ? | - |
? | |
| rne mRNA + H2O | the enzyme autoregulates its expression by cleavage and processing of its own rne mRNA | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 180000, unprocessed active enzyme, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoribonuclease RNase E | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
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