Application | Comment | Organism |
---|---|---|
medicine | extraordinarily long antisense RNAs of 3.5 and 7 kb protect a set of mRNAs from RNase E degradation that accumulate during phage infection. These antisense RNA-mRNA duplex formations mask single-stranded recognition sites of RNase E, leading to increased stability of the mRNAs. The interactions directly modulate RNA stability and provide an explanation for enhanced transcript abundance of certain mRNAs during phage infection | Prochlorococcus sp. |
Cloned (Comment) | Organism |
---|---|
recombinant overexpression of the His-tagged enzyme in Escherichia coli | Prochlorococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimum concentration 12 mM | Prochlorococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prochlorococcus sp. | - |
- |
- |
Prochlorococcus sp. MED4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Prochlorococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | fluorogenic cleavage assay | Prochlorococcus sp. | ? | - |
? | |
additional information | fluorogenic cleavage assay | Prochlorococcus sp. MED4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase E | - |
Prochlorococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Prochlorococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Prochlorococcus sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | extraordinarily long antisense RNAs of 3.5 and 7 kb protect a set of mRNAs from RNase E degradation that accumulate during phage infection. These antisense RNA-mRNA duplex formations mask single-stranded recognition sites of RNase E, leading to increased stability of the mRNAs. The interactions directly modulate RNA stability and provide an explanation for enhanced transcript abundance of certain mRNAs during phage infection | Prochlorococcus sp. |