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Literature summary for 3.1.26.12 extracted from

  • Khemici, V.; Poljak, L.; Luisi, B.F.; Carpousis, A.J.
    The RNase E of Escherichia coli is a membrane-binding protein (2008), Mol. Microbiol., 70, 799-813.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
N305D the mutation is localized in the catalytic domain of RNase E Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane localized to the inner cytoplasmic membrane, segment-A is necessary and sufficient for RNase E binding to membranes Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
464000
-
co-sedimentation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli KSL2000
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
RNase E is renatured on the Ni-NTA resin by decreasing the concentration of urea to 0.8 M Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9SA RNA + H2O 9Sa is a fragment of the 9S precursor of 5S rRNA Escherichia coli ?
-
?
9SA RNA + H2O 9Sa is a fragment of the 9S precursor of 5S rRNA Escherichia coli KSL2000 ?
-
?
RNA + H2O
-
Escherichia coli ?
-
?
RNA + H2O
-
Escherichia coli KSL2000 ?
-
?

Subunits

Subunits Comment Organism
tetramer co-sedimentation Escherichia coli

Synonyms

Synonyms Comment Organism
RNase E RNase E is an essential endoribonuclease involved in RNA processing and mRNA degradation Escherichia coli