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3.1.26.12: ribonuclease E

This is an abbreviated version!
For detailed information about ribonuclease E, go to the full flat file.

Word Map on EC 3.1.26.12

Reaction

endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions =

Synonyms

Ams/Rne/Hmp1 polypeptide, AqaRng, endoribonuclease E, endoribonuclease RNase E, More, NCgl2281, ribonuclease E, RNase E, RNase E/G, RNase E/G-type endoribonuclease, RNase ES, RNase EV, RNaseE, Rne, Rne protein, RneC, Rng, SSO1404, SynRne

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.12 ribonuclease E

Turnover Number

Turnover Number on EC 3.1.26.12 - ribonuclease E

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.015
5' hydroxylated fluorogenic oligonucleotide
-
pH 7.5, 25°C, recombinant N-terminal domain
-
0.014
5' monophosphorylated fluorogenic oligonucleotide
-
pH 7.5, 25°C, recombinant N-terminal domain
-
1.4
5'-GGGACAGUAUUUG-3'
-
in 25 mM bis-Tris-propane (pH 8.3), 15 mM MgCl2, 100 mM NaCl, 0.1% (v/v) Triton X-100, and 1 mM dithiothreitol
0.192
AAUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.15
CAUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.765
GAUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.121
GUUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.209
UAUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.015
UUUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.023
UUUUU-DN34-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C