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3.1.26.12: ribonuclease E

This is an abbreviated version!
For detailed information about ribonuclease E, go to the full flat file.

Word Map on EC 3.1.26.12

Reaction

endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions =

Synonyms

Ams/Rne/Hmp1 polypeptide, AqaRng, endoribonuclease E, endoribonuclease RNase E, More, NCgl2281, ribonuclease E, RNase E, RNase E/G, RNase E/G-type endoribonuclease, RNase ES, RNase EV, RNaseE, Rne, Rne protein, RneC, Rng, SSO1404, SynRne

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.12 ribonuclease E

Cloned

Cloned on EC 3.1.26.12 - ribonuclease E

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli
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expressed as a fusion with an N-terminal His6 tag in Escherichia coli strain BL21 (DE3)
expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DG5alpha and BL21(DE3) cells
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expressed in Escherichia coli DH5alpha cells
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expressed in Escherichia coli JM109 cells
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expressed in Escherichia coli strain BZ99
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expression in wild-type rne cells and in thermosensitive mutant rne-50 and rne-3071 cells, overexpression of truncated 110 kDa mutant enzyme in rne50 cells
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expression of His-tagged full-length wild-type RNase E and His-tagged N-terminal ribonucleolytic domain RTD-RNase E, residues 1-498, in strain BL21(DE3)
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expression of His-tagged N-terminal catalytic domain in strain BL21(DE3)
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expression of His-tagged truncated enzyme mutants in Escherichia coli strain BL21(DE3), complementation of rne null mutation of Escherichia coli strain KSL2000
expression of His-tagged truncated enzyme mutants in strain BL21(DE3), complementation of rne null mutation of strain KSL2000
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expression of His-tagged wild-type and mutant catalytic domains
expression of N-terminal enzyme half, comprising residues 1-499, as C-terminally His6- and Myc-tagged or maltose-binding protein-fused protein in strain BL21(DE3)
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expression of N-terminally His6-tagged wild-type and mutant full-length enzymes, and of isolated N-terminally His6-tagged S1 domain comprising residues 35-125
expression of the His-tagged N-terminal RNaseE catalytic N domain, comprising residues 1-529, in strain BL21(DE3)
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expression of wild-type and mutant enzyme and N-terminal catalytic domains in Escherichia coli
expression of wild-type and mutant enzymes
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expression of wild-type and mutant enzymes in different modified strains, cell growth curves, overview
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FLAG-tagged RNase E is expressed in Escherichia coli BL21(DE3) cells
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fragments of RNase E, either containing the enolase-binding microdomain (residues 833-851), the PNPase-binding site (residues 844-1061) or both (residues 734-1061)
gene rne, expression of His-tagged N-terminal half of RNase E in strain BL21(DE3)
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gene rne, expression of the isolated, His-tagged catalytic domain Rne498, comprising residues 1-498, in strain BL21(DE3)
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gene rne, expression of wild-type and mutant enzymes in Escherichia coli and in the Saccharomyces cerevisiae two-hybrid system
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gene rne, overexpression in Escherichia coli, functional complementation of the RNase E-deficient strain CJ1832 of Escherichia coli
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gene rne, overexpression in strain BL21(DE3)
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gene rne, overexpression in strain BL21(DE3) as His-tagged enzyme
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gene rne-1
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gene rng, overexpression of His10-tagged RNase E/G, Rng, in Escherichia coli strain BL21(DE3)
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oligohistidine-tagged polypeptides corresponding to the N-terminal half of RNase E with wild-type or mutant sequences
overexpressed in Escherichia coli ER2566
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overexpression of active His- and Myc-tagged truncated enzyme in strain BL21(DE3)
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overexpression of His10-tagged enzyme in Escherichia coli strain BL21(DE3)
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overexpression of plasmid-encoded rne gene increases cell doubling time and can lead to plasmid loss or aquisition of mutations that reduce RNase E activity
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overexpression of wild-type and truncation mutant enzymes in strain Bl21(DE3), expression in the yeast two-hybrid system, overview
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recombinant expression of FLAG-tagged truncation mutants
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recombinant overexpression of the His-tagged enzyme in Escherichia coli
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rne gene amplified and subcloned into pCR-BluntII-TOPO vector to generate pTOPOS14Rne. Expression plasmid pBADS14Rne constructed by excising the rne gene as a 3.3 kb gene Nco I-Pme I fragment from pTOPOS14Rne and inserting the fragment into the medium copy number plasmid pBADlacZ, putting RNase E expression under the control of the arabinose inducible promoter PBAD, and transformed into Escherichia coli AC23 (rne-1). RNase E putative enolase-binding microdomain (residues 885-909) and the putative PNPase-binding microdomain from S14 RNase E (residues 1015-1094) cloned