3.1.26.12: ribonuclease E
This is an abbreviated version!
For detailed information about ribonuclease E, go to the full flat file.
Word Map on EC 3.1.26.12
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3.1.26.12
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degradosome
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polynucleotide
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phosphorylase
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pnpase
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srnas
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endonuclease
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hfq
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helicase
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endonucleolytic
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exoribonuclease
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single-stranded
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enolase
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stem-loops
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polya
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rna-binding
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polycistronic
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ribonucleolytic
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e-dependent
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base-pairing
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dead-box
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autoregulation
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e-mediated
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e-like
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ompa
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endoribonucleolytic
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5'-terminal
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intercistronic
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cole1-type
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monophosphorylated
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5'-monophosphorylated
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shine-dalgarno
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rho-independent
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hfq-dependent
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glucosamine-6-phosphate
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riboswitches
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au-rich
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glm
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rna-processing
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crescentus
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analysis
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medicine
- 3.1.26.12
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degradosome
- polynucleotide
- phosphorylase
- pnpase
- srnas
- endonuclease
- hfq
- helicase
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endonucleolytic
- exoribonuclease
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single-stranded
- enolase
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stem-loops
- polya
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rna-binding
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polycistronic
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ribonucleolytic
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e-dependent
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base-pairing
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dead-box
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autoregulation
-
e-mediated
-
e-like
- ompa
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endoribonucleolytic
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5'-terminal
-
intercistronic
-
cole1-type
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monophosphorylated
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5'-monophosphorylated
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shine-dalgarno
-
rho-independent
-
hfq-dependent
- glucosamine-6-phosphate
- riboswitches
-
au-rich
- glm
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rna-processing
- crescentus
- analysis
- medicine
Reaction
endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions =
Synonyms
Ams/Rne/Hmp1 polypeptide, AqaRng, endoribonuclease E, endoribonuclease RNase E, More, NCgl2281, ribonuclease E, RNase E, RNase E/G, RNase E/G-type endoribonuclease, RNase ES, RNase EV, RNaseE, Rne, Rne protein, RneC, Rng, SSO1404, SynRne
ECTree
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KM Value
KM Value on EC 3.1.26.12 - ribonuclease E
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0.00033
5' hydroxylated fluorogenic oligonucleotide
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pH 7.5, 25°C, recombinant N-terminal domain
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0.00023
5' monophosphorylated fluorogenic oligonucleotide
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pH 7.5, 25°C, recombinant N-terminal domain
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0.00207
5'-GGGACAGUAUUUG-3'
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in 25 mM bis-Tris-propane (pH 8.3), 15 mM MgCl2, 100 mM NaCl, 0.1% (v/v) Triton X-100, and 1 mM dithiothreitol
0.00073
AAUUU-containing RNA oligonucleotide
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recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00046
CAUUU-containing RNA oligonucleotide
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recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00124
GAUUU-containing RNA oligonucleotide
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recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00037
GUUUU-containing RNA oligonucleotide
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recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00081
UAUUU-containing RNA oligonucleotide
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recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00028
UUUUU-containing RNA oligonucleotide
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recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00032
UUUUU-DN34-containing RNA oligonucleotide
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recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00013
5'-hydroxylated BR13 with multiple single-stranded regions
0.0036
5'-GGGACAGUAUUUG
5'-monophosphorylated BR13 with one single-stranded site
additional information
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kinetics of the reaction with 5' monophosphorylated RNA substrates
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additional information
additional information
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kinetics of tRNA precursor cleavage at 37°C and 44°C
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additional information
additional information
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kinetics, purified recombinant His-tagged N-terminal catalytic domain
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