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3.1.26.12: ribonuclease E

This is an abbreviated version!
For detailed information about ribonuclease E, go to the full flat file.

Word Map on EC 3.1.26.12

Reaction

endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions =

Synonyms

Ams/Rne/Hmp1 polypeptide, AqaRng, endoribonuclease E, endoribonuclease RNase E, More, NCgl2281, ribonuclease E, RNase E, RNase E/G, RNase E/G-type endoribonuclease, RNase ES, RNase EV, RNaseE, Rne, Rne protein, RneC, Rng, SSO1404, SynRne

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.12 ribonuclease E

KM Value

KM Value on EC 3.1.26.12 - ribonuclease E

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00033
5' hydroxylated fluorogenic oligonucleotide
-
pH 7.5, 25°C, recombinant N-terminal domain
-
0.00023
5' monophosphorylated fluorogenic oligonucleotide
-
pH 7.5, 25°C, recombinant N-terminal domain
-
0.008
5'-GAGACAGUAUUUG
5'-hydroxylated LU13
0.00013 - 0.0036
5'-GGGACAGUAUUUG
0.00207
5'-GGGACAGUAUUUG-3'
-
in 25 mM bis-Tris-propane (pH 8.3), 15 mM MgCl2, 100 mM NaCl, 0.1% (v/v) Triton X-100, and 1 mM dithiothreitol
0.00073
AAUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00046
CAUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00124
GAUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00037
GUUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00081
UAUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00028
UUUUU-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
0.00032
UUUUU-DN34-containing RNA oligonucleotide
-
recombinant enzyme N-terminal half, pH 7.5, 30°C
additional information
additional information
-