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Literature summary for 3.1.26.12 extracted from

  • Ow, M.C.; Kushner, S.R.
    Initiation of tRNA maturation by RNase E is essential for cell viability in E. coli (2002), Genes Dev., 16, 1102-1115.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.12

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in different modified strains, cell growth curves, overview Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of the rneDELTA645 allele with an introduced stop codon, the mutant strain shows reduced mRNA decay compared to rne wild-type or overexpressing strains Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of tRNA precursor cleavage at 37°C and 44°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
9S rRNA + H2O Escherichia coli the enzyme is essential for 9S RNA processing ?
-
 ?
additional information Escherichia coli initiation of tRNA 5' maturation by RNase E is essential for cell viability, the enzyme initiates the processing of polycistronic RNA of several operons, e.g. of glyW cysT leuZ, argX hisR leuT proM, or lysT valT lysW valZ lysY lysZlysQ, as well as of monocistronic transcripts such as pheU, pheV, asnT, asnU, asnV, or asnW, mapping of cleavage sites at the 3' end within tRNA precursors, overview, the enzyme is essential for degradation of many mRNAs, e.g. of rpsO ?
-
 ?
tRNAAsn precursor + H2O Escherichia coli
-
 ?
-
 ?
tRNAHis precursor + H2O Escherichia coli
-
 ?
-
 ?
tRNAPhe precursor + H2O Escherichia coli
-
 ?
-
 ?
tRNAPro precursor + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 several strains, overview, gene rne
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 RNA degradation activity in wild-type and mutant strains, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9S rRNA + H2O
-
 Escherichia coli ?
-
 ?
9S rRNA + H2O the enzyme is essential for 9S RNA processing Escherichia coli ?
-
 ?
additional information initiation of tRNA 5' maturation by RNase E is essential for cell viability, the enzyme initiates the processing of polycistronic RNA of several operons, e.g. of glyW cysT leuZ, argX hisR leuT proM, or lysT valT lysW valZ lysY lysZlysQ, as well as of monocistronic transcripts such as pheU, pheV, asnT, asnU, asnV, or asnW, mapping of cleavage sites at the 3' end within tRNA precursors, overview, the enzyme is essential for degradation of many mRNAs, e.g. of rpsO Escherichia coli ?
-
 ?
tRNAAsn precursor + H2O
-
 Escherichia coli ?
-
 ?
tRNAHis precursor + H2O
-
 Escherichia coli ?
-
 ?
tRNAPhe precursor + H2O
-
 Escherichia coli ?
-
 ?
tRNAPro precursor + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase E
-
 Escherichia coli