1.1.1.79: glyoxylate reductase (NADP+)

This is an abbreviated version, for detailed information about glyoxylate reductase (NADP+), go to the full flat file.

Reaction

glycolate
+
NADP+
=
glyoxylate
+
NADPH
+
H+

Synonyms

AtGR1, AtGR2, D-2-hydroxy-acid dehydrogenase, glyoxylate reductase, glyoxylate reductase 1, glyoxylate reductase 2, glyoxylate reductase isoform 1, glyoxylate reductase/hydroxypyruvate reductase, GLYR1, GLYR2, GOR1, GR/HPR, GR1, GR2, GRHPR, GRHRP, More, TthGR1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.79 glyoxylate reductase (NADP+)

Cloned

Cloned on EC 1.1.1.79 - glyoxylate reductase (NADP+)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells
expressed as GFP-fusion protein in tobacco BY-2 cells; expressed as GFP-fusion protein in tobacco BY-2 cells; expressed as His-tag fusion protein in E. coli BL-21(DE3) Rosetta (pLysS), full-length and truncated GR2 sequences introduced into Escherichia coli, only the recombinant truncated GR2 is soluble, expression markedly improved by co-expression of the GroES/GroEL chaperone; expressed in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells and in Nicotiana tabacum BY-2 cells
expressed in Escherichia coli
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL and Escherichia coli B834(DE3)pRARE
-
expressed in Escherichia coli Top10 as His-tag fusion protein
-
expression in Escherichia coli strain JM109
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli
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expression of the Myc-His9-tagged enzyme in Escherichia coli
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ORF YNL274c, DNA and amino acid sequence determination and analysis, expression in yeast strains, overview
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