BRENDA - Enzyme Database show
show all sequences of 1.1.1.79

A preliminary account of the properties of recombinant human glyoxylate reductase (GRHPR), LDHA and LDHB with glyoxylate, and their potential roles in its metabolism

Mdluli, K.; Booth, M.P.; Brady, R.L.; Rumsby, G.; Biochim. Biophys. Acta 1753, 209-216 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli strain JM109
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Homo sapiens
0.011
-
NADPH
37C; pH 7.5, 37C, recombinant enzyme
Homo sapiens
0.058
-
Hydroxypyruvate
37C, cofactor: NAPDH; pH 7.5, 37C, recombinant enzyme, with NADPH
Homo sapiens
0.19
-
Hydroxypyruvate
37C, cofactor: NADH; pH 7.5, 37C, recombinant enzyme, with NADH
Homo sapiens
0.24
-
glyoxylate
37C, cofactor: NADPH; pH 7.5, 37C, recombinant enzyme, with NADPH
Homo sapiens
1
-
glyoxylate
37C, cofactor: NADH; pH 7.5, 37C, recombinant enzyme, with NADH
Homo sapiens
2.42
-
NADH
37C; pH 7.5, 37C, recombinant enzyme
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Homo sapiens
5829
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NAD(P)H
Homo sapiens
the enzyme plays a protective role in detoxification of glyoxylate
glycolate + NAD(P)+
-
-
?
hydroxypyruvate + NAD(P)H
Homo sapiens
-
D-glycerate + NAD(P)+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
; recombinant
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NAD(P)H
-
667814
Homo sapiens
glycolate + NAD(P)+
-
-
-
?
glyoxylate + NAD(P)H
the enzyme plays a protective role in detoxification of glyoxylate
667814
Homo sapiens
glycolate + NAD(P)+
-
-
-
?
glyoxylate + NADH
affinity for NADPH is lower than affinity for NADH
667814
Homo sapiens
glycolate + NAD+
-
-
-
?
glyoxylate + NADPH
-
667814
Homo sapiens
glycolate + NADP+
-
-
-
?
hydroxypyruvate + NAD(P)H
-
667814
Homo sapiens
D-glycerate + NAD(P)+
-
-
-
?
hydroxypyruvate + NAD(P)H
preferred substrate
667814
Homo sapiens
D-glycerate + NAD(P)+
-
-
-
?
hydroxypyruvate + NADH
affinity for NADPH is lower than affinity for NADH
667814
Homo sapiens
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADPH
-
667814
Homo sapiens
D-glycerate + NADP+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
27
-
glyoxylate
37C, cofactor: NADPH; pH 7.5, 37C, recombinant enzyme, with NADPH
Homo sapiens
38
-
Hydroxypyruvate
37C, cofactor: NAPDH; pH 7.5, 37C, recombinant enzyme, with NADPH
Homo sapiens
65
-
Hydroxypyruvate
37C, cofactor: NADH; pH 7.5, 37C, recombinant enzyme, with NADH
Homo sapiens
67
-
glyoxylate
37C, cofactor: NADH; pH 7.5, 37C, recombinant enzyme, with NADH
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
; higher affinity for NADPH
Homo sapiens
NADPH
lower affinity for NADH; preferred cofactor
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli strain JM109
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
; higher affinity for NADPH
Homo sapiens
NADPH
lower affinity for NADH; preferred cofactor
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Homo sapiens
0.011
-
NADPH
37C; pH 7.5, 37C, recombinant enzyme
Homo sapiens
0.058
-
Hydroxypyruvate
37C, cofactor: NAPDH; pH 7.5, 37C, recombinant enzyme, with NADPH
Homo sapiens
0.19
-
Hydroxypyruvate
37C, cofactor: NADH; pH 7.5, 37C, recombinant enzyme, with NADH
Homo sapiens
0.24
-
glyoxylate
37C, cofactor: NADPH; pH 7.5, 37C, recombinant enzyme, with NADPH
Homo sapiens
1
-
glyoxylate
37C, cofactor: NADH; pH 7.5, 37C, recombinant enzyme, with NADH
Homo sapiens
2.42
-
NADH
37C; pH 7.5, 37C, recombinant enzyme
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Homo sapiens
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NAD(P)H
Homo sapiens
the enzyme plays a protective role in detoxification of glyoxylate
glycolate + NAD(P)+
-
-
?
hydroxypyruvate + NAD(P)H
Homo sapiens
-
D-glycerate + NAD(P)+
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NAD(P)H
-
667814
Homo sapiens
glycolate + NAD(P)+
-
-
-
?
glyoxylate + NAD(P)H
the enzyme plays a protective role in detoxification of glyoxylate
667814
Homo sapiens
glycolate + NAD(P)+
-
-
-
?
glyoxylate + NADH
affinity for NADPH is lower than affinity for NADH
667814
Homo sapiens
glycolate + NAD+
-
-
-
?
glyoxylate + NADPH
-
667814
Homo sapiens
glycolate + NADP+
-
-
-
?
hydroxypyruvate + NAD(P)H
-
667814
Homo sapiens
D-glycerate + NAD(P)+
-
-
-
?
hydroxypyruvate + NAD(P)H
preferred substrate
667814
Homo sapiens
D-glycerate + NAD(P)+
-
-
-
?
hydroxypyruvate + NADH
affinity for NADPH is lower than affinity for NADH
667814
Homo sapiens
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADPH
-
667814
Homo sapiens
D-glycerate + NADP+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
27
-
glyoxylate
37C, cofactor: NADPH; pH 7.5, 37C, recombinant enzyme, with NADPH
Homo sapiens
38
-
Hydroxypyruvate
37C, cofactor: NAPDH; pH 7.5, 37C, recombinant enzyme, with NADPH
Homo sapiens
65
-
Hydroxypyruvate
37C, cofactor: NADH; pH 7.5, 37C, recombinant enzyme, with NADH
Homo sapiens
67
-
glyoxylate
37C, cofactor: NADH; pH 7.5, 37C, recombinant enzyme, with NADH
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
Other publictions for EC 1.1.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739242
Zong
Up regulation of glyoxylate re ...
Homo sapiens
Pathol. Res. Pract.
212
365-371
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722899
Ching
Glyoxylate reductase isoform 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
54
152-168
2012
-
-
1
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
4
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
696125
Allan
Role of plant glyoxylate reduc ...
Arabidopsis thaliana
Biochem. J.
423
15-22
2009
-
-
-
-
-
-
1
-
2
-
-
-
-
1
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2
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1
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1
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1
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2
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685774
Ogino
Characterization of recombinan ...
Thermus thermophilus, Thermus thermophilus HB27
Biotechnol. Prog.
24
321-325
2008
-
-
2
-
-
-
3
8
-
-
1
2
10
2
-
-
2
-
-
-
-
-
5
1
2
-
4
8
1
1
-
4
-
-
-
-
-
2
4
-
-
-
-
3
-
8
-
-
1
2
10
-
-
2
-
-
-
-
5
1
2
-
4
8
1
1
-
-
-
-
-
-
-
-
688090
Simpson
Identification and characteriz ...
Arabidopsis thaliana
J. Exp. Bot.
59
2545-2554
2008
-
-
1
-
2
-
1
4
2
-
4
4
-
7
-
-
1
-
-
1
-
4
15
1
-
-
-
4
1
1
-
2
-
2
-
-
-
2
4
-
2
-
-
1
-
4
2
-
4
4
-
-
-
1
-
1
-
4
15
1
-
-
-
4
1
1
-
2
-
-
-
-
-
-
688091
Allan
Gamma-hydroxybutyrate accumula ...
Arabidopsis thaliana, Nicotiana tabacum
J. Exp. Bot.
59
2555-2564
2008
-
-
-
-
-
-
2
-
2
-
-
5
-
5
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
2
-
-
5
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684141
Yoshikawa
Structure of archaeal glyoxyla ...
Pyrococcus horikoshii OT3
Acta Crystallogr. Sect. D
63
357-365
2007
-
-
1
1
-
-
-
4
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
-
-
4
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
4
-
-
2
-
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-
-
1
-
-
-
-
4
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
685902
Hoover
Kinetic mechanism of a recombi ...
Arabidopsis thaliana
Can. J. Bot.
85
896-902
2007
-
-
1
-
-
-
3
2
-
-
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-
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
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-
1
8
-
-
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-
1
1
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3
8
2
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-
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-
1
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-
2
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-
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-
-
690207
Rintala
The ORF YNL274c (GOR1) codes f ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
Yeast
24
129-136
2007
-
-
1
-
1
-
-
-
-
-
-
2
-
21
-
-
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-
3
-
6
-
1
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1
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2
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1
2
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1
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2
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-
3
-
6
-
1
-
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-
1
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-
-
-
-
-
-
-
-
669886
Booth
Structural basis of substrate ...
Homo sapiens
J. Mol. Biol.
360
178-189
2006
-
-
1
1
4
-
1
-
-
-
-
3
-
2
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-
1
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2
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5
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1
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2
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1
2
1
4
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1
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3
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1
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2
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5
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1
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-
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-
-
-
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667814
Mdluli
A preliminary account of the p ...
Homo sapiens
Biochim. Biophys. Acta
1753
209-216
2005
-
-
1
-
-
-
-
7
1
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-
2
-
1
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1
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8
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1
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4
1
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2
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1
2
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7
1
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2
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1
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8
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1
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4
1
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669351
Genolet
Promoter rearrangements cause ...
Homo sapiens, Mus musculus
J. Biol. Chem.
280
24143-24152
2005
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1
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5
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2
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2
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2
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287318
Kleczkowski
-
Kinetics and regulation of the ...
Spinacia oleracea
Z. Naturforsch. C
50
21-28
1995
1
-
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15
4
1
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1
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1
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1
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1
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2
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1
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2
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15
-
4
1
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1
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1
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1
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287319
Husic
NADH:hydroxypyruvate reductase ...
Chlamydomonas reinhardtii
Arch. Biochem. Biophys.
252
396-408
1987
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-
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1
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1
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1
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1
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1
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2
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1
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1
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1
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1
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287320
Kleczkowski
Purification and characterizat ...
Glycine max, Spinacia oleracea, Triticum aestivum
Biochem. J.
239
653-659
1986
-
-
-
-
-
-
-
4
-
-
2
3
-
3
-
-
1
-
-
1
3
-
3
1
-
-
-
-
1
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-
3
-
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-
3
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4
-
-
2
3
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1
-
1
3
-
3
1
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-
-
-
1
-
-
-
-
-
-
-
-
-
287321
Yokota
Purification and some properti ...
Euglena gracilis
Biochem. J.
227
211-216
1985
-
-
-
-
-
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4
2
3
-
1
1
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3
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1
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1
1
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4
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1
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2
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2
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4
-
2
3
-
1
1
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1
-
1
1
-
4
-
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1
-
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-
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-
287322
Mulligan
Effects of glyoxylate on photo ...
Spinacia oleracea
Plant Physiol.
72
415-419
1983
-
-
-
-
-
-
-
1
1
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286409
Yokota
Occurrence and subcellular dis ...
Euglena gracilis
Agric. Biol. Chem.
45
15-22
1981
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287324
Fukuda
Purification and properties of ...
Neurospora crassa
Agric. Biol. Chem.
45
1153-1158
1981
-
-
-
-
-
-
2
4
-
-
1
1
-
1
-
-
1
-
-
-
1
1
4
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
4
-
-
1
1
-
-
-
1
-
-
1
1
4
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
287325
Fukuda
Purification and enzymatic pro ...
Saccharomyces cerevisiae
J. Biochem.
87
841-846
1980
-
-
-
-
-
-
4
4
-
-
-
1
-
1
-
-
1
-
-
-
1
1
3
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
4
-
-
-
1
-
-
-
1
-
-
1
1
3
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
287326
Sagisaka
Occurrence of nicotinamide ade ...
Populus gelrica
Plant Physiol.
65
377-381
1980
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
1
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287327
Yokota
Occurrence and properties of t ...
Euglena gracilis
Biochem. J.
184
189-192
1979
-
-
-
-
-
-
-
2
3
-
-
1
-
5
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
3
-
-
1
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287328
Tochikura
Purification and properties of ...
Saccharomyces cerevisiae
J. Biochem.
86
105-110
1979
-
-
-
-
-
-
10
2
-
-
1
1
-
1
-
-
1
-
-
-
1
1
5
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
10
-
2
-
-
1
1
-
-
-
1
-
-
1
1
5
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
287329
Zeltich
Effect of glycidate, an inhibi ...
Nicotiana tabacum
Plant Physiol.
61
236-241
1978
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-